{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T18:09:29Z","timestamp":1769882969482,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The aim of this study is to further clarify the material basis and mechanism of the QianLieXin (QLX) capsule. UPLC\u2010Q\u2010MS was employed to identify the active substances absorbed into the blood and prostate. Pharmacological network analysis and anti\u2010inflammatory assays were performed. Twenty principal bioactive compounds of the QLX capsule were identified in the blood and prostate tissues of rats. Network pharmacology and molecular docking analyses demonstrated 292 potential targets of these active substances relevant to the treatment of chronic prostatitis. Among them, chlorogenic acid, apigenin, kaempferol, isoquercitrin, and ursolic acid were identified as the primary agents exerting anti\u2010inflammatory effects. The principal molecular targets included AKT1, EGFR, PIK3, and MAPK, among others. Key signaling pathways encompassed the neuroligin\u2010receptor interaction pathway as well as lipid metabolism and atherosclerosis\u2010related pathways. Experimental results demonstrated that both the medicated serum and the active substances significantly suppressed lipopolysaccharide\u2010induced interleukin\u20101\u03b2 levels, inhibited the expression of NF\u2010\u03baB protein, and reduced reactive oxygen species production, indicating notable anti\u2010inflammatory and antioxidant activities. Furthermore, QLX has a strong binding effect on targets such as EGFR, AKT, and MMP9. Medicated serum was shown to downregulate the EGFR\/AKT\/MAPK\/MMP9 signaling pathways. Our findings clarified the pharmacodynamic substances of the multicomponent QLX capsule and its regulatory mechanism.<\/jats:p>","DOI":"10.1002\/bmc.70256","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:39:37Z","timestamp":1767926377000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010Inflammatory Pharmacodynamic Basis and Mechanism of QianLieXin Capsule in Chronic Prostatitis: Insights From Absorbed Components and Network Pharmacology"],"prefix":"10.1002","volume":"40","author":[{"given":"Cejia","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Shandong University  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linghe","family":"Zang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine Shandong Hongjitang Pharmaceutical Group Co. Ltd  Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Qin","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiqi","family":"Wen","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Ma","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruihua","family":"Sui","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Sun","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahang","family":"Yu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoqing","family":"Meng","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine Shandong Hongjitang Pharmaceutical Group Co. 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