{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:41:31Z","timestamp":1787636491350,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A novel reverse\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) method for validating the amount of HES in dissolving microneedle patches (DMNs) loaded with HES\u2010based nanostructured lipid carriers (HES\u2010NLC)s has been developed. The method was developed and validated according to the guidelines published by the International Council for Harmonization (ICH), using a mobile phase of methanol:water (90:10\u2009v\/v) at a flow rate of 0.5\u2009mL\/min, with UV detection at 284\u2009nm. This method demonstrated accuracy from 94% to 108%, with a precision of 2% across all evaluated concentrations, along with high selectivity, sensitivity, and stability for transdermal application studies. The method also showed good linearity in rat plasma and skin homogenate, with no interference from biological matrices, which aligns with FDA guidelines. Permeation studies revealed enhanced HES delivery via DMNs with nearly 20\u2010fold higher drug deposition compared to passive diffusion. Pretreatment with DMNs significantly boosted the transdermal permeation of HES (112.86\u2009\u00b1\u200923.58\u2009\u03bcg\/cm\n                    <jats:sup>2<\/jats:sup>\n                    after 24\u2009h,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), by increased flux (1.31\u2009\u00b1\u20090.11\u2009\u03bcg\/cm\n                    <jats:sup>2<\/jats:sup>\n                    \/h) and permeability coefficient (0.36\u2009\u00d7\u200910\n                    <jats:sup>\u2212<\/jats:sup>\n                    6\u2009cm\/s) values. This validated method offers a promising tool for advancing transdermal evaluation in innovative pharmaceutical formulations.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70257","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T13:21:26Z","timestamp":1763126486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["RP\u2010HPLC\u2013Validated Method for Hesperidin Estimation in Rat Skin Following Transdermal Delivery via Dissolving Microneedle Patch"],"prefix":"10.1002","volume":"39","author":[{"given":"Gopika","family":"Gopan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences Nitte (deemed to be University)  Mangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2815-5384","authenticated-orcid":false,"given":"Jobin","family":"Jose","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences Nitte (deemed to be University)  Mangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kartik Bhairu","family":"Khot","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences Nitte (deemed to be University)  Mangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"issue":"2","key":"e_1_2_7_2_1","first-page":"220","article-title":"Qualitative and Quantitative Estimation of Hesperidin in Peel and Juice of Citrus Fruits by RP\u2010HPLC Method Growing in Kurdistan Region\/Iraq","volume":"33","author":"Adham A. N.","year":"2015","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787\u2010025\u201001696\u2010z"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094\u2010024\u201003258\u2010z"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010022\u201004146\u2010y"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2024.124293"},{"key":"e_1_2_7_7_1","first-page":"2738","volume-title":"AIP Conference Proceedings","author":"Cahyono B.","year":"2023"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2023.130205"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202403210"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26175377"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122609"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010020\u201003950\u20108"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.140948"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010023\u201004283\u2010y"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2024.162037"},{"issue":"7","key":"e_1_2_7_16_1","first-page":"527","article-title":"Research Progress of Synthesis and Biological Activity of Hesperidin and Hesperitin Derivatives","volume":"42","author":"Huang A.","year":"2018","journal-title":"Progress in Pharmaceutical Sciences"},{"issue":"96","key":"e_1_2_7_17_1","first-page":"27463","article-title":"International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human use ICH Harmonised Tripartite Guideline Validation of Analytical Procedures: Text and Methodology Q2(R1)","volume":"62","author":"ICH","year":"1997","journal-title":"Federal Register"},{"key":"e_1_2_7_18_1","first-page":"1","volume-title":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use: Validation of Analytical Procedures","author":"ICH Q2 (R2)","year":"2022"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.019"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40824\u2010021\u201000226\u20106"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.110541"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.33263\/LIANBS122.050"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmaf016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.13647"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2298\/vsp1401060k"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7ay00051k"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S415728"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.117749"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1571"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S474034"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e25598"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2022.12.004"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ipha.2024.09.005"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010025\u201004404\u20109"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.25004\/ijpsdr.2023.150511"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2023.100718"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315198"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3426"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.138855"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jics.2024.101521"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/01932691.2020.1844739"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.05.001"},{"key":"e_1_2_7_43_1","first-page":"1","volume-title":"Bioanalytical Method Validation Guidance for Industry","author":"U.S. Department of Health and Human Services, Food and Drug Administration","year":"2018"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70257","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T12:52:41Z","timestamp":1764420761000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,14]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/bmc.70257"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70257","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,14]]},"assertion":[{"value":"2025-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70257"}}