{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T04:45:34Z","timestamp":1770957934956,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rise of inflammation\u2010 and age\u2010related diseases reveals a need for safer therapies, with medicinal plants offering promising bioactive compounds for treatment. This study used chromatographic and NMR tools along with UHPLC\u2010ESI\u2010LIT\u2010Orbitrap\u2010MS to analyze the phytochemical profile of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Euphorbia greenwayi<\/jats:italic>\n                    <\/jats:styled-content>\n                    's main fractions (EG1:EG5), which showed promising dose\u2010dependent inhibition of COX\u20101, COX\u20102, and LOX enzymes through multivariate data analysis. A total of 125 metabolites were identified via UHPLC\u2010ESI\u2010LIT\u2010Orbitrap\u2010MS, including nine isolated and NMR\u2010structurally confirmed compounds, representing diverse chemical classes including phenolic acids and flavonoid glycosides as key components. Furthermore, this study utilized multivariate statistical methods, including Hierarchical Clustering Analysis (HCA) and Orthogonal Projection to Latent Structures Discriminant Analysis (OPLS\u2010DA), to classify solvent fractions based on their chemical composition. The results demonstrated significant dose\u2010dependent inhibitory effects of cyclooxygenase COX\u20101, COX\u20102, and lipoxygenase (LOX) enzymes. Notably, Fraction EG5 exhibited potent antiaging effects, as demonstrated by its inhibitory activity on collagenase and elastase enzymes. Further investigation revealed bioactive metabolites, such as xanthurenic acid, tricin, and quercetin, contributing to the anti\u2010inflammatory and antiaging properties. These findings provide valuable insights into the therapeutic potential of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. greenwayi<\/jats:italic>\n                    <\/jats:styled-content>\n                    , particularly for anti\u2010inflammatory and antiaging applications, and lay a foundation for future pharmacological studies on its bioactive compounds.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70268","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:05:36Z","timestamp":1764547536000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comprehensive UHPLC\u2010ESI\u2010Orbitrap\u2010MS Profiling and Bioactivity\u2010Guided Fractionation of\n                    <scp>\n                      <i>Euphorbia greenwayi<\/i>\n                    <\/scp>\n                    : Mechanistic Insights Into Anti\u2010Inflammatory and Antiaging Bioactive Metabolites"],"prefix":"10.1002","volume":"40","author":[{"given":"Ahmed\u00a0F.","family":"Essa","sequence":"first","affiliation":[{"name":"Chemistry of Natural Compounds Department National Research Centre  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai\u00a0M.","family":"Elghonemy","sequence":"additional","affiliation":[{"name":"Chemistry of Natural Compounds Department National Research Centre  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmaa\u00a0S.","family":"Abd\u00a0Elkarim","sequence":"additional","affiliation":[{"name":"Chemistry of Tanning Materials and Leather Technology Department National Research Centre  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeinab\u00a0A.","family":"El\u2010Gendy","sequence":"additional","affiliation":[{"name":"Pharmacology Department, Medical and Clinical Research Institute National Research Centre  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naglaa\u00a0M.","family":"Ammar","sequence":"additional","affiliation":[{"name":"Therapeutic Chemistry Department National Research Centre  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reham\u00a0S.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Alexandria University  Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania\u00a0F.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Chemistry of Natural Compounds Department National Research Centre  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed\u00a0A.","family":"Farag","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3302-3623","authenticated-orcid":false,"given":"Abdelsamed\u00a0I.","family":"Elshamy","sequence":"additional","affiliation":[{"name":"Chemistry of Natural Compounds Department National Research Centre  Giza Egypt"},{"name":"Faculty of Pharmaceutical Sciences Tokushima Bunri University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-57288-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2025.103067"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-006-0228-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17091129"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmy057"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27196470"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3923\/jbs.2010.138.144"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1008881"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.009258"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.129008"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-017-0983-1"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2506"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf062314x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations8050062"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12061263"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28114304"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24132412"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2018.01.021"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24203763"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2025.114418"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152970"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0111"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3803"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-9007-5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf304002h"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27072314"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf201501x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dib.2018.12.008"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.02.132"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26165055"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo10100403"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf025995e"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01502-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b00270"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmw016"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2021.08.029"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules171214821"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.5459"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e14569"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29030735"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.12.041"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.09.092"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-012-0665-8"},{"key":"e_1_2_7_45_1","first-page":"129","volume-title":"Advanced Protocols in Oxidative Stress II","author":"Petrovic N.","year":"2009"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3263"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.17795\/jjnpp-4861"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10010131"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1601100227"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0321018"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b06929"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22010174"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400541j"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201200733"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.03.015"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.02.030"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.03.017"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14956"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.22146\/ijc.79819"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2015.01.005"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14114454"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200942"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111755"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e17305"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43094-024-00599-0"},{"key":"e_1_2_7_66_1","first-page":"515","article-title":"Phytochemical Analysis of Euphorbia greenwayi Aerial Parts: Antioxidant and Anti\u2010Inflammatory Potential","volume":"67","author":"Zaghlol A.","year":"2024","journal-title":"Egyptian Journal of Chemistry"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12858-018-0095-7"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2022.2096078"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.130642"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8228"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:49:41Z","timestamp":1766072981000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,30]]},"references-count":69,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/bmc.70268"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70268","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,30]]},"assertion":[{"value":"2025-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70268"}}