{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:52:07Z","timestamp":1766073127024,"version":"3.48.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104358"],"award-info":[{"award-number":["82104358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2021423066"],"award-info":[{"award-number":["H2021423066"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To screen for anti\u2010PMO active components in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cornus officinalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    based on ER\u03b2 enzyme activity using affinity ultrafiltration and molecular docking techniques.ER\u03b2 enzyme and UHPLC\u2010Q\u2010Exactive Orbitrap MS were employed to analyze the anti\u2010PMO activity and predict the components of different concentrations of ethanol extracts of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. officinalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Molecular docking was used to verify the interaction mechanism between small molecule ligands and ER\u03b2. The anti\u2010PMO activity of the predicted components was further verified using MC3T3\u2010L1 cells. The results showed that the ER\u03b2 enzyme can serve as a target enzyme for screening anti\u2010PMO components. The 50% ethanol extract exhibited the best activity. UHPLC\u2010Q\u2010Exactive Orbitrap MS identified and analyzed 17 potential active components. Based on the binding rate and molecular docking results, the following three active components were identified: sweroside, 4\u2010hydroxycinnamic acid, and cornuside. In\u00a0vitro activity validation confirmed that these components have potential anti\u2010PMO effects. The ER\u03b2 enzyme can be used as a potential target enzyme for screening anti\u2010PMO active components in traditional Chinese medicine. The combination of affinity ultrafiltration and molecular docking provides an effective and rapid method for active component screening, offering valuable references for the targeted therapy, mechanism exploration, and quality control of traditional Chinese medicine.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70270","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T08:00:49Z","timestamp":1764316849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening of ER\u03b2\u2010Targeted Antipostmenopausal Osteoporosis Chemical Constituents From\n                    <scp>\n                      <i>Cornus officinalis<\/i>\n                    <\/scp>\n                    Based on Affinity Ultrafiltration and UHPLC\u2010Q\u2010Exactive Orbitrap MS"],"prefix":"10.1002","volume":"40","author":[{"given":"Yawen","family":"Li","sequence":"first","affiliation":[{"name":"Jilin 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