{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:32:38Z","timestamp":1786977158984,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T00:00:00Z","timestamp":1765238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Preeclampsia (PE) is a severe hypertensive disorder of pregnancy involving complex metabolic disturbances. This study aimed to elucidate PE's pathogenesis and aspirin's preventive mechanism using metabolomics and network pharmacology. The untargeted urinary metabolomics via ultra\u2010performance liquid chromatography\u2010quadrupole time\u2010of\u2010flight mass spectrometry (UPLC\u2010Q\u2010TOF\u2010MS) were performed on healthy pregnant women (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911), PE patients (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20099), and potential PE patients with aspirin intervention (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912). Multivariate analysis identified 162 differential metabolites. The PE group exhibited a distinct metabolic profile characterized by dysregulation in tryptophan, purine, arachidonic acid, and quinone pathways, contributing to increased oxidative stress, vasoconstriction, platelet aggregation, and inflammation. Notably, aspirin intervention reversed the levels of 28 key metabolites. Its primary preventive mechanism was associated with the targeted modulation of tryptophan metabolism and one\u2010carbon pool by folate, which appeared to inhibit the serotonin synthesis pathway and improve endothelial function. Importantly, both metabolomics and network pharmacology identified the one\u2010carbon metabolic and tryptophan metabolism pathways as key preventive targets for PE, corroborating their role in aspirin's mechanism. In conclusion, this study revealed a \u201cmetabolic network imbalance\u201d in PE and confirmed that aspirin conferred specific protection to metabolism. These findings offered new insights for multitarget prevention strategies for PE and the development of diagnostic systems for metabolic biomarkers.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70284","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T11:12:26Z","timestamp":1765278746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Study on the Pathogenesis of Preeclampsia and the Mechanism of Aspirin Prevention by Metabolomics"],"prefix":"10.1002","volume":"40","author":[{"given":"Bai","family":"Yutao","sequence":"first","affiliation":[{"name":"Chromatography and Mass Spectrometry Technology Platform, Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xin","sequence":"additional","affiliation":[{"name":"Chromatography and Mass Spectrometry Technology 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