{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:54:42Z","timestamp":1766073282928,"version":"3.48.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zuclopenthixol was reported to treat schizophrenia, psychoses, and acute bipolar mania. 2\u2010Chlorothioxanthon is reported to be zuclopenthixol official Impurity C (IMP C), and it is considered to be zuclopenthixol synthetic precursor. IMP C was reported to have toxicity on mRNA and protein expression. Environmentally validated HPTLC and HUPLC were developed for the simultaneous analysis of zuclopenthixol and IMP C in the analytical standard form and in pharmaceutical form, according to ICH guidelines. Regarding the HPTLC method, zuclopenthixol and its toxic impurity were separated using a mobile phase consisting of dichloromethane:ethyl acetate:triethylamine (6:4.5:0.4, v\/v), and the scanning wavelength was 257\u2009nm. For the HUPLC method, the simultaneous separation was completed within 2\u2009min using Waters, CORTECS C\n                    <jats:sub>8<\/jats:sub>\n                    (4.6\u2009mm\u2009\u00d7\u2009150, 2.7\u2009\u03bcm) as a stationary phase. The applied mobile phase was an isocratic mixture of methanol:water (80:20, v\/v), pH was 3.25\u2009\u00b1\u20090.02 using orthophosphoric acid, pumping at 3\u2009mL\/min, and the detection wavelength was 257\u2009nm. To evaluate the greenness value of the presented methods, the Eco\u2010Scale Assessment method, Analytical Greenness Metric, and Green Analytical Procedure Index were applied. The practicality of the analytical methods was evaluated using the Blue Applicability Grade Index. Additionally, the analytical whiteness was determined via the RGB algorithm.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70286","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T10:53:43Z","timestamp":1764932023000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Greenness, Blueness, and Whiteness Assessment of Novel Chromatographic Methods for Separation and Simultaneous Analysis of Zuclopenthixol and Its Major Potentially Toxic Impurity: A Comparative Study"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7032-9149","authenticated-orcid":false,"given":"Fatma\u00a0F.","family":"Abdallah","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-2490","authenticated-orcid":false,"given":"Eglal\u00a0A.","family":"Abdelaleem","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3498-017X","authenticated-orcid":false,"given":"Aml\u00a0G.","family":"Korany","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0799-7934","authenticated-orcid":false,"given":"Noha\u00a0H.","family":"Amin","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4527-8885","authenticated-orcid":false,"given":"Ahmed\u00a0M.","family":"Gouda","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13065\u2010024\u201001339\u20104"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13065\u2010025\u201001391\u20108"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MICROC.2025.113326"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4025"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1556\/1006.2016.29.6.9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13065\u2010025\u201001572\u20105"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13065\u2010025\u201001598\u20109"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/S13206\u2010024\u201000144\u20104"},{"volume-title":"British Pharmacopeia","year":"2023","key":"e_1_2_9_10_1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/B617536H"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD001164"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.SAA.2024.125409"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20106080(05)80189\u20108"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.TRAC.2013.04.010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570\u20100232(03)00576\u20102"},{"volume-title":"Proceedings of the international conference on harmonization of technical requirements for registration of pharmaceuticals for human use, Geneva, Switzerland","year":"2006","author":"Guideline, I. 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