{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:54:03Z","timestamp":1766073243457,"version":"3.48.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003654","name":"Korea Environmental Industry and Technology Institute","doi-asserted-by":"publisher","award":["2021003320006"],"award-info":[{"award-number":["2021003320006"]}],"id":[{"id":"10.13039\/501100003654","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1I1A3059197"],"award-info":[{"award-number":["2021R1I1A3059197"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n To develop an LC\u2013MS\/MS method for simultaneous quantification of thioridazine and its metabolites in rat plasma, samples were prepared by protein precipitation and filtration using thioridazine\u2010d3 as an internal standard. Chromatographic separation was achieved on a reversed\u2010phase C18 UPLC column with 0.1% formic acid in acetonitrile and 10\u2010mM ammonium acetate in water at 0.7\u2009mL\/min. Quantification was performed using multiple reaction monitoring in positive ion mode with the following precursor\u2013product ion transitions: m\/z 371.1\u2009\u2192\u2009126.1 (thioridazine), m\/z 387.1\u2009\u2192\u2009126.1 (mesoridazine), m\/z 403.1\u2009\u2192\u2009126.1 (thioridazine\u20102\u2010sulfone), m\/z 387.2\u2009\u2192\u2009126.1 (thioridazine\u20105\u2010sulfoxide), and 374.1\u2009\u2192\u2009129.1 (thioridazine\u2010d3). This method showed good linearity: 0.1\u20131000\u2009ng\/mL for thioridazine (\n r<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.9980) and 0.5\u20131000\u2009ng\/mL for mesoridazine (\n r<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.9981), thioridazine\u20102\u2010sulfone (\n r<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.9976), and thioridazine\u20105\u2010sulfoxide (\n r<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.9967). The accuracy of the LLOQ and QC samples for thioridazine and its metabolites was within 85%\u2013115%, with intrabatch and interbatch precisions (CV%) below 15%. The average recovery ranged from 98% to 109%, and no significant matrix effect was observed, with values ranging from 93% to 110%. All analytes were stable under the conditions used in this study. This method was applied in a pharmacokinetic study following the intravenous administration of thioridazine to rats.\n <\/jats:p>","DOI":"10.1002\/bmc.70298","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T07:40:46Z","timestamp":1765784446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Determination of Thioridazine and Its Metabolites by UHPLC\u2013MS\/MS With Application to a Pharmacokinetic Study in Rats"],"prefix":"10.1002","volume":"40","author":[{"given":"Kyung\u2010Sun","family":"Moon","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Sciences, College of Pharmacy Kangwon National University Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong\u2010Min","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Sciences, College of Pharmacy Kangwon National University Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5937-2146","authenticated-orcid":false,"given":"Sung\u2010Hoon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Sciences, College of Pharmacy Kangwon National University Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.6.1049"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.5.505"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009\u20109236(96)80010\u20105"},{"key":"e_1_2_9_5_1","first-page":"246","article-title":"Phenothiazine Treatment in Acute Schizophrenia; Effectiveness: The National Institute of Mental Health Psychopharmacology Service Center Collaborative Study Group","volume":"10","author":"Guttmacher M. S.","year":"1964","journal-title":"Archives of General Psychiatry"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009\u20109236(96)90150\u20102"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401\u2010023\u201001102\u2010w"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691\u2010200008000\u201000006"},{"issue":"4","key":"e_1_2_9_9_1","first-page":"171","article-title":"Application of Liquid Chromatography Coupled With Mass Spectrometry (LC\/MS) to Determine Antidepressants in Blood Samples","volume":"58","author":"Pufal E.","year":"2008","journal-title":"Archives of Forensic Medicine and Criminology"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2019.4715"},{"issue":"2","key":"e_1_2_9_11_1","first-page":"138","article-title":"Stability Indicating Method Development and Validation of FIMASARTAN by Reverse\u2010Phase High\u2010Performance Liquid Chromatography in Bulk and Pharmaceutical Dosage Form","volume":"14","author":"Sruthi A.","year":"2021","journal-title":"Asian Journal of Pharmaceutical and Clinical Research"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010023\u201001190\u20105"},{"volume-title":"Bioanalytical Method Validation: Guidance for Industry","year":"2018","author":"U.S. Food and Drug Administration","key":"e_1_2_9_13_1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.03.105"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T15:48:51Z","timestamp":1766072931000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,14]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/bmc.70298"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70298","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2025,12,14]]},"assertion":[{"value":"2025-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70298"}}