{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:44:28Z","timestamp":1790286268889,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Endophytes are microorganisms that reside inside the living tissues of leaves and possess antimicrobial, antioxidant, anticancer and anti\u2010inflammatory activity etc. Liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) represents a powerful analytical platform for comprehensive metabolomic profiling of endophytic fungi, offering superior sensitivity, selectivity and structural elucidation capabilities compared to conventional methods. This study employed LC\u2013MS\/MS and GC\u2013MS analyses to characterise bioactive compounds from the methanolic extract of endophytic fungus\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Acremonium sclerotigenum<\/jats:italic>\n                    <\/jats:styled-content>\n                    CBS 124.42 isolated from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Stevia rebaudiana<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves. LC\u2013MS\/MS analysis identified nine major bioactive compounds, including flavonoid glycosides (928.4\u2009m\/z), rutin derivatives (1454.7\u2009m\/z), cyclic depsipeptides (1445.4\u2009m\/z), cyanidin\u20103\u2010O\u2010rutinoside (595.3\u2009m\/z), macrolactams (1394.2\u2009m\/z), proanthocyanins (1232.8\u2009m\/z), oligomeric imperatorin (676.8\u2009m\/z) and cyanidin\u20103\u2010O\u2010glucoside (1232.8\u2009m\/z). Stevioside was detected at 9.02\u2009min with characteristic fragments at 317.9\u2009m\/z. GC\u2013MS analysis revealed 18 distinct compounds, with benzene (azidomethyl) being most abundant (100% relative area), followed by hydrazine carbothioamide (71.3%) and benzyl(1,2,3\u2010thiadiazol\u20104\u2010yl) carbamate (43.22%). Significantly, this study reports the first identification of hydrazine carbothioamide and benzyl(1,2,3\u2010thiadiazol\u20104\u2010yl) carbamate from natural sources, compounds previously known only from chemical synthesis. These compounds demonstrated significant bioactivities including antioxidant (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200928.1\u2009\u00b1\u20096.96\u2009mg\/g), antimicrobial (zone of inhibition: 10.3\u201323.6\u2009mm) and anticancer properties, establishing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. sclerotigenum<\/jats:italic>\n                    <\/jats:styled-content>\n                    CBS 124.42 as a novel source of therapeutically relevant secondary metabolites.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70326","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T08:26:57Z","timestamp":1765787217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["LC\u2013MS\/MS and GC\u2013MS Analysis\u2010Based Novel Bioactive Compounds From Endophyte\n                    <scp>\n                      <i>Acremonium sclerotigenum<\/i>\n                    <\/scp>\n                    CBS 124.42 Isolated From\n                    <scp>\n                      <i>Stevia rebaudiana<\/i>\n                    <\/scp>\n                    (Bertoni) Bertoni"],"prefix":"10.1002","volume":"40","author":[{"family":"Nishant","sequence":"first","affiliation":[{"name":"Dept. of Bioengineering and Biotechnology BIT Mesra  Ranchi Jharkhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Chandra","sequence":"additional","affiliation":[{"name":"Dept. of Bioengineering and Biotechnology BIT Mesra  Ranchi Jharkhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1050453"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2023.2175366"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b02616"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.33176\/AACB-19-00023"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.4162\/nrp.2007.1.4.279"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo8020031"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-024-01207-1"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40199-024-00530-1"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/hydrobiology1030024"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2011.0316"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-023-04087-2"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40345-024-00338-7"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/10426507.2022.2136665"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.33696\/pathology.4.043"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmz006"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13389-6"},{"key":"e_1_2_13_18_1","first-page":"98","article-title":"Phytochemical, Antimicrobial and Bio\u2010Active Component Analysis of \n                     Platycerium superbum (L.) 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