{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T19:08:05Z","timestamp":1769886485437,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cerebral ischemia\u2013reperfusion injury (CIRI) is classified under the category of stroke. Sanhua Decoction (SHD) is a classic prescription for the treatment of stroke, but its pharmacodynamic material basis and mechanism of action have not been fully elucidated. This study aimed to elucidate the bioactive components and therapeutic mechanisms of SHD against CIRI by integrating serum pharmacochemistry, network pharmacology, and metabolomics. Using ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight tandem mass spectrometry, a 22 absorbed SHD prototype compounds were identified from rat serum. Through the application of network pharmacology and molecular docking technology, it was concluded that the key active ingredients, such as marmesin and aloe\u2010emodin, mainly play a therapeutic role through key targets such as steroid receptor coactivator (SRC) and protein kinase B1 (AKT1). Molecular docking showed that these targets had strong affinity with key compounds. The results of pharmacodynamics showed that SHD could improve the neurological function score of CIRI rats, reduce the infarct area, and improve the pathological changes of brain tissue. Metabolomics analysis showed that SHD may play a therapeutic role by regulating steroid hormone synthesis and ubiquinone\u2010related pathways. This study provides a methodological framework for exploring the role of Chinese herbal compounds in the treatment of CIRI.<\/jats:p>","DOI":"10.1002\/bmc.70339","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T04:16:26Z","timestamp":1767672986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of Sanhua Decoction Against Cerebral Ischemia\u2013Reperfusion Injury by Serum Pharmacochemistry, Network Pharmacology, and Metabolomics"],"prefix":"10.1002","volume":"40","author":[{"given":"Tingting","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"An","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Bioequivalence Research of Generic Drug Evaluation, NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine Shenzhen Institute for Drug Control  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010li","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"},{"name":"Guizhou Inheritance Base of Traditional Chinese Medicine Processing Technology  Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiyao","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4401-5469","authenticated-orcid":false,"given":"Yan","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxin","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"},{"name":"Guizhou Inheritance Base of Traditional Chinese Medicine Processing Technology  Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Sui","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Bioequivalence Research of Generic Drug Evaluation, NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine Shenzhen Institute for Drug Control  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Bioequivalence Research of Generic Drug Evaluation, NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine Shenzhen Institute for Drug Control  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044\u2010015\u20101350\u20108"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq154"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.16588\/j.cnki.issn1002\u20101108.2019.04.002"},{"issue":"14","key":"e_1_2_12_5_1","first-page":"4079","article-title":"Effects of Sanhua Decoction on Expression of Sodium Ion Channel in Cerebral Infarct Zone in Rats","volume":"5","author":"Dai Y. 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Department of Pharmacology Gannan Medical College.\u201d 42."},{"issue":"11","key":"e_1_2_12_12_1","first-page":"2790","article-title":"Based on the Expression of NLRP6, the Effect of miR\u20109 on Nerve Injury Repair and TGF\u2010\u03b2\/Smad Expression in Rats With Cerebral Ischemia\u2010Reperfusion Was Explored","volume":"44","author":"Kong B.","year":"2024","journal-title":"Chinese Journal of Gerontology"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.14066\/j.cnki.cn21\u20101349\/r.2021.1290"},{"issue":"66","key":"e_1_2_12_14_1","article-title":"Baicalein Amelioratescerebral Ischemia\u2010Reperfusion Injury by Inhibiting Ferroptosis via Regulating GPX4\/ACSL4\/ACSL3 Axis","volume":"13","author":"Li M.","year":"2023","journal-title":"Chemico\u2010Biological Interactions"},{"issue":"12","key":"e_1_2_12_15_1","first-page":"67","article-title":"Research Progress of Sanhua Decoction in the Treatment of Stroke","volume":"34","author":"Li W. 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