{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T18:42:14Z","timestamp":1769884934976,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study aims to evaluate the in\u00a0vivo drug interactions between sotorasib and ondansetron to provide critical data for accidental or self\u2010medication cases to ensure safe and effective concurrent use. A rapid UHPLC\u2010MS\/MS technique was developed on a Zorbax Eclipse XDB\u2010C18 column (3.0\u2009\u00d7\u2009100\u2009mm, 3.5\u2009\u03bcm) using ammonium bicarbonate and methanol. The MRM analysis identified distinct transitions for sotorasib, ondansetron, and the internal standard (IS) at\n                    <jats:italic>m\/z<\/jats:italic>\n                    561.2\u2009\u2192\u2009134.0, 317.0;\n                    <jats:italic>m\/z<\/jats:italic>\n                    294.0\u2009\u2192\u2009184.0, 170.0; and\n                    <jats:italic>m\/z<\/jats:italic>\n                    529.2\u2009\u2192\u2009326.1, 160.9, respectively. Target analytes were extracted from rat plasma by organic solvent precipitation. The UHPLC\u2010MS\/MS method revealed linearity as\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009&gt;\u20090.99, sensitivity at 0.4\u2009ng\/mL concentration, accuracy as 99.15%\u201399.87%, and precision as 1.62%\u20136.45% for LQC, MQC, and HQC. In silico predictive assessments revealed moderate and serious risk levels. Preclinical pharmacokinetic study revealed that ondansetron exhibited diminished C\n                    <jats:sub>max<\/jats:sub>\n                    (0.69\u2010fold), AUC (0.45\u2010fold), and half\u2010life (0.75\u2010fold), alongside increased clearance (2.04\u2010fold) when administered concurrently. Study findings are likely due to CYP3A4 induction by sotorasib, overriding its P\u2010gp inhibition effects. To alleviate these effects, physicians must consider dose adjustments or stringent alternative antiemetic strategies.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70345","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T03:58:59Z","timestamp":1767671939000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of the Novel, Fast Bioanalytical LC\u2010MS\/MS Technique for the Simultaneous Estimation of Sotorasib and Ondansetron: Application to In\u00a0Vivo Pharmacokinetic Assessments"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1871-9537","authenticated-orcid":false,"given":"Jony\u00a0Susanna","family":"Kandula","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-5414","authenticated-orcid":false,"given":"P.","family":"Radhakrishnanand","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC\u2010120028254"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/S11096\u2010016\u20100393\u20103"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.33483\/jfpau.1460173"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.12.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/FPHAR.2023.1310455\/FULL"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CRITREVONC.2005.03.007"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.20.00744"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.36468\/pharmaceutical-sciences.1145"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpdd.1501"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.9758\/cpn.22.1014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2024.107966"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00520\u2010017\u20103866\u20106"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e30437"},{"key":"e_1_2_8_15_1","unstructured":"FDA.2018. \u201cBioanalytical Method Validation: Guidance for Industry.\u201d Bioanalytical Method Validation May.1\u201344."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jradnu.2023.04.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(97)00198-2"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00780-4"},{"issue":"5","key":"e_1_2_8_19_1","first-page":"45","article-title":"Evaluating the Reliability of Drug Interaction Checkers in Psychiatric Prescription Safety: A Comparative Analysis","volume":"21","author":"Jd O. 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