{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T19:08:05Z","timestamp":1769886485505,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Microsorum fortunei<\/jats:italic>\n                    <\/jats:styled-content>\n                    (T. Moore) Ching (MF) is a traditional Chinese medicine utilized for the treatment of rheumatoid arthritis (RA). However, the chemical composition and underlying mechanisms of MF remain unclear. This study aims to identify the active ingredients of MF that contribute to its therapeutic effects on RA and to explore the associated mechanisms. A rat model of RA was established, and the effects of MF were evaluated by monitoring changes in appearance, body weight, inflammatory indices, joint histopathology, thymus and spleen indices, and levels of inflammatory factors. The ethyl acetate fraction of MF (EAF\u2010MF) was analyzed using UPLC\u2010Q\u2010TOF\/MS. Network pharmacology and molecular docking analyses were conducted to predict the anti\u2010inflammatory components and pathways. The EAF\u2010MF significantly alleviated RA symptoms, leading to reductions in foot swelling, arthritis index, joint damage and pro\u2010inflammatory cytokines. UPLC\u2010Q\u2010TOF\/MS identified 57 compounds within the EAF\u2010MF; network pharmacology identified 44 active components connecting 524 targets. The data showed that MF treatment of RA was related to oxytocin signaling pathway and Cushing syndrome. Molecular docking confirmed strong binding between core targets and key components. Consequently, this study elucidates the multi\u2010component, multi\u2010target, and multi\u2010pathway mechanisms of EAF\u2010MF in the treatment of RA, thereby supporting its clinical application.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70351","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T09:25:35Z","timestamp":1768209935000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Ethyl Acetate Fraction of\n                    <scp>\n                      <i>Microsorum fortunei<\/i>\n                    <\/scp>\n                    (T. Moore) Ching Alleviates Rheumatoid Arthritis via Multi\u2010Target Mechanisms: An Integrated Strategy Combining UPLC\u2010Q\u2010TOF\/MS, Network Pharmacology, and Experimental Validation"],"prefix":"10.1002","volume":"40","author":[{"given":"Jinxi","family":"Wang","sequence":"first","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Qin","sequence":"additional","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanrong","family":"Tian","sequence":"additional","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongfang","family":"Ding","sequence":"additional","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guizhou University of Traditional Chinese Medicine  Guiyang Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guizhou University of Traditional Chinese Medicine  Guiyang Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2236-1275","authenticated-orcid":false,"given":"Guo","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guizhou University of Traditional Chinese Medicine  Guiyang Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9551-436X","authenticated-orcid":false,"given":"Shengju","family":"Pan","sequence":"additional","affiliation":[{"name":"Qiandongnan Miao and Dong Autonomous Prefecture Institute of Ethnic Medicine  Kaili Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.129291"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.112957"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70188"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201008187\u20106"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659\u2010021\u201000298\u2010w"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp2310178"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30193865"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24193475"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113610"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70069"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596\u2010024\u20102925\u20108"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc\u20102023\u20100119"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155903"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.132442"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.945129"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115939"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.15.55"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.864007"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adq0043"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1346054"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio\u20102018\u20100140"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14100706"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70186"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2025.102074"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.86677"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/00045632241285843"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787\u2010020\u201000688\u20105"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2025.102036"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels11020136"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2025.120387"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045539\u2010x"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3969\/J.ISSN.1000\u20105137.2023.04.005"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70191"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.02.115"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20210107.601"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.11.021"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.123.043391"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111598"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201055702\u2010z"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16020178"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70351","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T03:55:14Z","timestamp":1769831714000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,12]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/bmc.70351"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70351","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,12]]},"assertion":[{"value":"2025-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70351"}}