{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:41:37Z","timestamp":1791362497391,"version":"4.3.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Albiflorin and paeoniflorin are bioactive isomers found in\n                    <jats:italic>Paeoniae Radix Alba<\/jats:italic>\n                    , a key component of Traditional Chinese Medicine. Their accurate and simultaneous quantification is essential for pharmacological research. Standard separation methods often rely on LC\u2010MS using a mobile phase containing 0.1% formic acid. This study demonstrates that the use of 0.1% formic acid generates a secondary peak for albiflorin that exhibits an identical mass\u2010to\u2010charge ratio and similar fragmentation pattern as the main peak. To address this, an LC\u2010MS method was developed using a Kinetex phenyl\u2010hexyl column with a gradient elution using a buffered mobile phase of 10\u2009mM ammonium acetate and acetic acid (pH\u20094.4) in water and methanol. The method was validated for linearity, precision, accuracy, and sensitivity. It successfully separated paeoniflorin and albiflorin preventing the formation of the albiflorin artifact. The method demonstrated good linearity over a concentration range of 1\u201350\u2009\u03bcg\/mL. Applicability was tested through the analysis of a\n                    <jats:italic>Paeoniae Radix Alba<\/jats:italic>\n                    extract. The developed LC\u2010MS method enables accurate and simultaneous quantification of albiflorin and paeoniflorin by eliminating the formation of a second albiflorin peak. This makes the method potentially suitable for pharmacological studies of\n                    <jats:italic>Paeoniae Radix Alba<\/jats:italic>\n                    and other plants containing albiflorin and paeoniflorin.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70353","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:21:15Z","timestamp":1768803675000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Buffered LC\u2010MS Method for Resolving and Quantifying Albiflorin and Paeoniflorin"],"prefix":"10.1002","volume":"40","author":[{"given":"Alina","family":"Gazizova","sequence":"first","affiliation":[{"name":"Industrial and Analytical Chemistry, Department of Chemistry University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ela\u00a0H\u00fcmay","family":"Altincubuk","sequence":"additional","affiliation":[{"name":"Industrial and Analytical Chemistry, Department of Chemistry University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Oppermann","sequence":"additional","affiliation":[{"name":"Industrial and Analytical Chemistry, Department of Chemistry University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,18]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27238337"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10008"},{"key":"e_1_2_14_4_1","unstructured":"Dolan J. 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