{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:57:09Z","timestamp":1773863829086,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In the present study, preliminary in\u00a0vitro interaction between ET\u201026\u2010HCl and human serum albumin (HSA) was investigated, and the potential competitive binding effect from its major metabolite, ET\u201026\u2010acid, was evaluated. Rapid equilibrium dialysis (RED) was utilized under simulated physiological conditions (67 mM phosphate\u2010buffered saline solution, pH\u20097.4, 37 \u00b0C). After a methanol\u2010based deproteinization procedure, a validated HPLC\u2010UV method was employed to quantify post\u2010dialysis samples. It was found that the binding affinity of ET\u201026\u2010HCl to HSA was relatively lower than that of etomidate, with a binding association constant (\n                    <jats:italic>K<\/jats:italic>\n                    ) of 7.75\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    \u2009M\n                    <jats:sup>\u22121<\/jats:sup>\n                    and a protein binding rate (\n                    <jats:italic>PB<\/jats:italic>\n                    %) of 54.58%, respectively. Displacement experiments using warfarin and flurbiprofen as specific site markers revealed that ET\u201026\u2010HCl predominantly binds to Sudlow's Site I on HSA. Furthermore, ET\u201026\u2010acid was found to exhibit no significant competitive binding effect. These findings support ET\u201026\u2010HCl as a promising alternative to etomidate and warrant its further development for clinical anesthetic applications.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70362","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T13:59:01Z","timestamp":1769090341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Preliminary In\u00a0Vitro Interaction of ET\u201026\u2010HCl With Human Serum Albumin Through Rapid Equilibrium Dialysis Under Simulated Physiological Conditions"],"prefix":"10.1002","volume":"40","author":[{"given":"Wen","family":"You","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifei","family":"Bian","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuze","family":"Ji","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Sun","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Xiang","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0780-196X","authenticated-orcid":false,"given":"Dongying","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Analysis Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2023.115797"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.23614"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2019.1569625"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2374930"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31825fec26"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.13995"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.11.017"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.10039"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/57.2.156"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540500404093"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(83)90043\u20100"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.07.075"},{"key":"e_1_2_8_14_1","unstructured":"ICH Expert Working Group.2023. \u201cThe International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use Guidance for Industry Q2 (R2): Validation of Analytical Procedures.\u201d step 4 ICH Geneva. 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