{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:56:40Z","timestamp":1773863800446,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A sensitive and robust HPLC\u2010MS\/MS method was developed and validated for the simultaneous determination of YWS22026 and its major metabolite M3 in rat plasma using protein precipitation. Chromatographic separation was achieved on a Shim\u2010pack Scepter C18 column (4.6\u2009\u00d7\u200950\u2009mm, 3\u2009\u03bcm) with a gradient elution program (mobile phase A: 0.2% formic acid in water; mobile phase B: methanol) at a flow rate of 0.9\u2009mL\/min. Detection was performed via positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. The method demonstrated excellent specificity, accuracy (relative error, %RE &lt;\u20099.1%), precision (relative standard deviation, %RSD &lt;\u20099.9%), and linearity (5\u20132000\u2009ng\/mL for both analytes). The lower limit of quantification (LLOQ) was 5\u2009ng\/mL. Stability studies confirmed that plasma samples remained stable under various storage conditions (at room temperature for 10\u2009h, \u221220\u00b0C for 22\u2009days, three freeze\u2013thaw cycles, and 4\u00b0C autosampler storage for 73\u2009h). This validated method was successfully applied to a 26\u2010week concomitant toxicokinetic study in Sprague\u2013Dawley rats, which revealed significant sex\u2010related differences in exposure for both YWS22026 and M3. These findings provide valuable insights for informing the safety assessment of YWS22026 in subsequent clinical trials.<\/jats:p>","DOI":"10.1002\/bmc.70366","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:38:41Z","timestamp":1768804721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HPLC\u2010MS\/MS Method for the Simultaneous Quantification of SGLT2 Inhibitor YWS22026 and Its M3 Metabolite in Rat Plasma, With Application to Toxicokinetic Study"],"prefix":"10.1002","volume":"40","author":[{"given":"Zhiyuan","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Nanjing Tech University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Shao","sequence":"additional","affiliation":[{"name":"Jiangsu Center for Safety Evaluation of Drugs Jiangsu Provincial Institute of Materia Medica  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3460-0883","authenticated-orcid":false,"given":"Hongqun","family":"Qiao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Nanjing Tech University  Nanjing 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