{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:36:15Z","timestamp":1792427775394,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["2710086913"],"award-info":[{"award-number":["2710086913"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["2710086913 [KK\u20102505])"],"award-info":[{"award-number":["2710086913 [KK\u20102505])"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>N<\/jats:italic>\n                    \u2010(1,3\u2010Dimethylbutyl)\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2032\u2010phenyl\u2010\n                    <jats:italic>p<\/jats:italic>\n                    \u2010phenylenediamine (6\u2010PPD), a tire rubber antioxidant, and its oxidation product 6\u2010PPD\u2010quinone (6\u2010PPDQ) have raised environmental and health concerns due to their widespread occurrence and toxicity. This study aimed to develop and validate a sensitive liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) method for the simultaneous quantification of 6\u2010PPD and 6\u2010PPDQ in murine tissues. The method showed excellent linearity (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009\u2265\u20090.9977), low detection limits (0.056\u20130.967\u2009ng\/mL), and high accuracy and precision across multiple tissue matrices, enabling reliable quantification at trace levels. Following repeated intratracheal administration in mice, both analytes were detected in the liver, lungs, and kidneys, but not in the spleen. Notably, 6\u2010PPDQ exhibited marked dose\u2010dependent accumulation in the lungs, whereas 6\u2010PPD remained at low levels, suggesting strong pulmonary retention. In contrast, trace levels of 6\u2010PPD and a dose\u2010dependent increase in 6\u2010PPDQ were found in the kidneys, indicating renal elimination as a clearance route. These results reveal selective organ distribution, with the lung as the primary deposition site, and highlight 6\u2010PPDQ as a potential multi\u2010organ toxicant. The validated LC\u2013MS\/MS method provides a valuable analytical platform for future toxicokinetic, biomonitoring, and mechanistic studies of tire\u2010derived contaminants.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70367","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T10:14:44Z","timestamp":1769595284000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Determination of\n                    <i>N<\/i>\n                    \u2010(1,3\u2010Dimethylbutyl)\u2010\n                    <i>N<\/i>\n                    \u2032\u2010Phenyl\u2010\n                    <i>p<\/i>\n                    \u2010Phenylenediamine (6\u2010PPD) and 6\u2010PPD\u2010Quinone (6\u2010PPDQ) in Mouse Tissues After Intratracheal Administration Using a Validated LC\u2013MS\/MS Method"],"prefix":"10.1002","volume":"40","author":[{"given":"Moongi","family":"Ji","sequence":"first","affiliation":[{"name":"College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences Sunchon National University  Suncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byeongchan","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences Sunchon National University  Suncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u2010Hyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Jeonbuk Advanced Bio Research Korea Institute of Toxicology  Jeongeup Jeollabuk\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u2010Hein","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Jeonbuk Advanced Bio Research Korea Institute of Toxicology  Jeongeup Jeollabuk\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u2010Woong","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Jeonbuk Advanced Bio Research Korea Institute of Toxicology  Jeongeup Jeollabuk\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moonjung","family":"Hyun","sequence":"additional","affiliation":[{"name":"Center for Bio\u2010Health Research, Division of Gyeongnam Bio\u2010Environmental Research Korea Institute of Toxicology  Jinju Gyeongsangnam\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Jeonbuk Advanced Bio Research Korea Institute of Toxicology  Jeongeup Jeollabuk\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man\u2010Jeong","family":"Paik","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences Sunchon National University  Suncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c07376"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.2c00821"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.161836"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.3c02819"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.171220"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.164842"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2022.115922"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics12100733"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/urbansci9060228"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2024.109228"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2024.142830"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.2510150102"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics12060389"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd6951"}],"container-title":["Biomedical 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