{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:24:50Z","timestamp":1773390290129,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LH2021H097"],"award-info":[{"award-number":["LH2021H097"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fuzheng pill (FZP) is a traditional Chinese medicine formula with anti\u2010hepatocellular carcinoma (HCC) effect in clinical practice, but its therapeutic mechanism is still unclear. In this study, ultra\u2010performance liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry method was used to analyze the chemical composition of FZP, and combined with in\u00a0vivo and in\u00a0vitro experiments, to explore its potential mechanism of action in the treatment of liver cancer. A total of 236 components were identified in FZP. In\u00a0vivo experiments revealed that FZP suppressed tumor growth, improved immune function, and reversed liver and kidney injury caused by tumors, as shown by significant increases in the immune organ index, and that the levels of IFN\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    , IL\u20102, and TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    significantly increased after its administration. Serum biochemical indicators of liver and kidney function decreased. FZP inhibited the proliferation of HepG2 cells. Flow cytometry and Western blot results revealed that FZP modulated the expression levels of Bcl\u20102, Bax, Caspase\u20103, and cleaved Caspase\u20103, and promoted cell apoptosis. FZP may promote the apoptosis of tumor cells by activating the endogenous Bax\/Bcl\u20102\/cleaved Caspase\u20103 apoptosis pathway, which is mediated by mitochondria. These findings revealed the potential of FZP in the treatment of HCC and provided more treatment options for patients.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70384","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:21:56Z","timestamp":1771503716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Fuzheng Pill on Liver Cancer via the Mitochondrial Apoptosis Pathway"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7464-2160","authenticated-orcid":false,"given":"Shiwen","family":"Gao","sequence":"first","affiliation":[{"name":"Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine) Ministry of Education  Harbin People's Republic of 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