{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:24:25Z","timestamp":1773390265891,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Wine\u2010steaming of Cnidii Fructus is a traditional processing method believed to enhance efficacy, but its mechanistic basis remains unverified. This study integrated HPLC\u2010based chemical profiling with in\u00a0vivo anti\u2010osteoporotic evaluation in zebrafish to elucidate the effects of processing. Comparative fingerprinting and multicomponent quantification delineated distinct chemical profiles between raw Cnidii Fructus (RCF) and wine\u2010steamed Cnidii Fructus (WCF). Multivariate analysis (PCA, OPLS\u2010DA) pinpointed six differential coumarins. Quantitative analysis confirmed a marked decrease in imperatorin but increases in osthole, alloimperatorin, and xanthotoxol after processing. Critically, the WCF extract demonstrated superior efficacy over RCF in promoting bone mineralization and enhancing alkaline phosphatase (ALP) activity in an osteoporotic zebrafish model. The differential compounds counteracted dexamethasone\u2010induced impairment, with xanthotoxol showing the most potent osteogenic activity, followed by alloimperatorin, diosmin, osthole, and imperatorin. These findings establish that wine\u2010steaming potentiates the anti\u2010osteoporotic activity of WCF by modulating its coumarin composition, providing a chemical and pharmacological rationale for its traditional use and quality control.<\/jats:p>","DOI":"10.1002\/bmc.70390","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T11:53:30Z","timestamp":1771415610000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Strategy Integrating Fingerprint Analysis and Multicomponent Quantification for Quality Control and Anti\u2010Osteoporotic Activity Evaluation of Cnidii Fructus Before and After Processing by Wine Steaming"],"prefix":"10.1002","volume":"40","author":[{"given":"JiaYing","family":"Zou","sequence":"first","affiliation":[{"name":"First Affiliated Hospital Hebei University of Chinese Medicine  Shijiazhuang Hebei China"},{"name":"Graduate School Hebei University of Chinese Medicine  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4540-7339","authenticated-orcid":false,"given":"Hao","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang Hebei China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuchang","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang Hebei China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danrui","family":"Han","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang Hebei China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaona","family":"Wei","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital Hebei University of Chinese Medicine  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuhong","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang Hebei China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province  Shijiazhuang Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11657\u2010018\u20100505\u2010x"},{"key":"e_1_2_8_3_1","volume-title":"The Effect and Mechanism of Alloimperatorin Induced Apoptosis and Autophagy in Cervical Cancer Cells (Unpublished Doctoral Dissertation)","author":"Bai Y. 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