{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:24:22Z","timestamp":1773390262392,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trichosanthis Pericarpium<\/jats:italic>\n                    injection (TPI) is a Chinese patent medicine for coronary heart disease and stable angina pectoris. This study aimed to integrate UHPLC\u2010QE\u2010MS with fingerprint\u2013effect relationships, chemometrics, and spectrum\u2013effect relationship analyses to accurately screen active components and quantify quality markers in TPI. First, the impact of TPI on Ang II\u2010induced cardiomyocyte hypertrophy was assessed in\u00a0vitro, confirming its inhibitory effects on cardiac hypertrophy for the first time. Next, 57 compounds were identified using UHPLC\u2010QE\u2010MS. Twelve batches of TPI were characterized by UPLC\u2010UV, and these fingerprint spectra were correlated with their inhibitory effects on cardiac hypertrophy using Pearson correlation analysis and PLSR methods. Five key compounds were identified: trigonelline, adenine, adenosine, guanosine, and vanillic acid. Among them, except for adenosine, the four compounds showed significant anti\u2010hypertrophy activity. Finally, a rapid UHPLC\u2010QQQ\u2010MS\/MS quantification method revealed that trigonelline and guanosine had high content, minimal variation, and significant inhibitory effects, establishing them as suitable quality control indicators for TPI. This study provides valuable support for the pharmacodynamic material basis and quality control of\n                    <jats:italic>Trichosanthis Pericarpium<\/jats:italic>\n                    and TPI and for the quality evaluation of complex chemical ingredient products.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70397","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:26:26Z","timestamp":1772958386000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Quantitation of Cardiac Hypertrophy Inhibitory Components in\n                    <i>Trichosanthis Pericarpium<\/i>\n                    Injection Based on UHPLC\u2010QE\u2010MS and Spectrum\u2013Effect Relationships"],"prefix":"10.1002","volume":"40","author":[{"given":"Ziyan","family":"Bian","sequence":"first","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianlin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinguo","family":"Ding","sequence":"additional","affiliation":[{"name":"Shanghai No. 1 Biochemical &amp; Pharmaceutical Corporation Ltd  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Dong","sequence":"additional","affiliation":[{"name":"Shanghai No. 1 Biochemical &amp; Pharmaceutical Corporation Ltd  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengjie","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8670-7512","authenticated-orcid":false,"given":"Siqi","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4737-7564","authenticated-orcid":false,"given":"Miaomiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2358-5449","authenticated-orcid":false,"given":"Yuhong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1008-7683","authenticated-orcid":false,"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/hm9.0000000000000049"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477119"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2023.105328"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE\u201007\u20100619"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202200221"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehad655.3141"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2019.19.032"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440\u20101681.13848"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1995\u20107645(14)60328\u2010X"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118630"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.05.008"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.14148\/j.issn.1672\u20100482.2020.0607"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.4330\/wjc.v15.i12.633"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116337"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.20181715"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1009\u20100959.2022.04.020"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.045115"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115253"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40360\u2010023\u201000676\u20109"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22105063"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.13412\/j.cnki.zyyl.2019.05.016"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.16438\/j.0513\u20104870.2022\u20101123"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010022\u201000806\u20106"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1006\u20101533.2017.05.020"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2023.1130635"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3177"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1008\u2010049X.2018.10.018"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.16438\/j.0513\u20104870.2018\u20100302"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70397","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70397","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70397","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:42:33Z","timestamp":1773380553000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,6]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/bmc.70397"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70397","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,6]]},"assertion":[{"value":"2025-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70397"}}