{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:23:57Z","timestamp":1773390237191,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study reports the first bioanalytical method for simultaneous estimation of Tramadol HCl and dexketoprofen trometamol in rat plasma using Tapentadol as an internal standard. A validated LC\u2010MS\/MS method was developed following USFDA guidelines. Analytes were extracted from plasma by protein precipitation technique using acetonitrile. Chromatographic separation was achieved on a Waters Symmetry Shield RP\u201018 column (250\u2009\u00d7\u20094.6\u2009mm, 5\u2009\u03bcm) with an isocratic mobile phase of acetonitrile and 0.1% formic acid in HPLC grade water (30:70 v\/v) at a flow rate of 1.0 mL\/min, yielding a 7\u2010min runtime. Detection was performed using electrospray ionization with ion transitions of m\/z 222.3398\u2009\u2192\u200970.0307 for Tapentadol, m\/z 264.4351\u2009\u2192\u200996.4527 for Tramadol HCl, and m\/z 376.4239\u2009\u2192\u2009100.4520 for dexketoprofen trometamol. The method showed good accuracy, with mean recoveries of 92.79%\u201398.15% for Tramadol HCl and 91.78%\u201398.37% for dexketoprofen trometamol. Excellent linearity was obtained, with\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    values of 0.99983 (22.5\u2013900\u2009ng\/mL) and 0.99963 (7.5\u2013300\u2009ng\/mL), respectively. All validation parameters met acceptable criteria. The method is suitable for evaluating pharmacokinetic parameters that indicate drug efficacy and safety.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70398","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T01:59:11Z","timestamp":1771552751000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Robust LC\u2010MS\/MS Bioanalytical Strategy for Simultaneous Quantification of Tramadol HCl and Dexketoprofen Trometamol and Its Use in Pharmacokinetic Studies"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8052-5127","authenticated-orcid":false,"given":"Adusumalli\u00a0Srinivasa","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Chemistry Vignan's Foundation for Science Technology and Research  Guntur AP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Bangaraiah","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Vignan's Foundation for Science Technology and Research  Guntur AP India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8250-3570","authenticated-orcid":false,"given":"Sathish\u00a0Kumar","family":"Konidala","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Biotechnology and Pharmaceutical Sciences Vignan's Foundation for Science Technology and Research  Guntur AP India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4172\/2157\u20107064.1000152"},{"issue":"3","key":"e_1_2_10_3_1","article-title":"Spectrophotometric Methods for Simultaneous Estimation of Tramadol Hydrochloride and Diclofenac Sodium in Combined Dosage Form","volume":"6","author":"Akhtar J.","year":"2013","journal-title":"Research Journal of Pharmacy and Technology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations8020021"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.03.019"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4088\/JCP.22m14734"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010011\u20100539\u20103"},{"issue":"6","key":"e_1_2_10_8_1","first-page":"3324","article-title":"HPTLC Determination of Dexketoprofen and Thiocolchicoside","volume":"4","author":"Bhavnani V. S.","year":"2012","journal-title":"Journal of Chemical and Pharmaceutical Research"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.114147"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a032540"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations10060365"},{"key":"e_1_2_10_12_1","unstructured":"ChemicalBook.2024. \u201cTramadol Hydrochloride: Chemical Properties and Solubility.\u201d ChemicalBook Database. Accessed February 11 2026.https:\/\/www.chemicalbook.com."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2019.10.005"},{"key":"e_1_2_10_14_1","first-page":"842","article-title":"Spectrophotometric Determination of Dexketoprofen and Thiocolchicoside","volume":"4","author":"Choudhari V. 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