{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:49:16Z","timestamp":1789044556801,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82560709"],"award-info":[{"award-number":["82560709"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aims to develop an ultra high\u2010performance liquid chromatography\u2013mass spectrometry (UPLC\u2010MS\/MS) method to quantify clobazam, perampanel, and lacosamide plasma concentrations in pediatric epilepsy patients. Elution was performed using a gradient elution program, with a mobile phase composed of 0.01% formic acid in water and 0.01% formic acid in acetonitrile. The flow rate was 0.6\u2009mL\/min. The injector temperature was 10\u00b0C. The injection volume was 4\u2009\u03bcL. Linearity, sensitivity, precision, accuracy, specificity, carryover, and extraction recovery were evaluated. Therapeutic drug monitoring data of 1132 samples were analyzed. The method was linear within 10\u2013600\u2009ng\/mL, 100\u20132000\u2009ng\/mL, and 1.0\u201330.0\u2009\u03bcg\/mL for clobazam, perampanel, and lacosamide, respectively (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009\u2265\u20090.998). The results indicated that the within\u2010run and between\u2010run precision coefficient of variation (CV %) did not exceed 15.0%, and that the accuracy (bias) ranged from \u22128.3% to 13.6%. Recovery ranged from 90.9% to 108.1%. All plasma samples could be maintained for up to 3\u2009h at ambient temperature, 24\u2009h at 4\u00b0C, 30\u2009days at \u221230\u00b0C, and after successive freeze\u2013thaw cycles in the absence of significant degradation. We successfully developed a simple and rapid LC\u2010MS\/MS method for the simultaneous measurement of three new generation ASMs, and successfully applied it to clinical practice.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70403","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:15:32Z","timestamp":1773008132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Drug Monitoring of Clobazam, Perampanel, and Lacosamide Using a UPLC\u2010MS\/MS Method: Analysis of 5\u2010Year Experience in a Large Cohort of Pediatric Epilepsy Patients From China"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3502-6043","authenticated-orcid":false,"given":"Ting","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy Xinjiang Medical University  Xinjiang China"},{"name":"Department of Pharmacy People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"},{"name":"Institute of Clinical Pharmacy of Xinjiang Uygur Autonomous Region, People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"},{"name":"Institute of Clinical Pharmacy of Xinjiang Uygur Autonomous Region, People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Xinjiang Hospital of Beijing Children's Hospital, Children's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"},{"name":"Institute of Clinical Pharmacy of Xinjiang Uygur Autonomous Region, People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"},{"name":"Institute of Clinical Pharmacy of Xinjiang Uygur Autonomous Region, People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology, Xinjiang Hospital of Beijing Children's Hospital, Children's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u2010Hai","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"},{"name":"Institute of Clinical Pharmacy of Xinjiang Uygur Autonomous Region, People's Hospital of Xinjiang Uygur Autonomous Region  Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmsacl.2022.04.005"},{"key":"e_1_2_11_3_1","unstructured":"Bioanalytical Method Validation Guidance for Industry|FDA.2025.https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/bioanalyticalmethod\u2010validation\u2010guidance\u2010industry (accessed June 12 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