{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:24:11Z","timestamp":1773390251468,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K10089"],"award-info":[{"award-number":["25K10089"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Valganciclovir (VGCV) is the first\u2010line drug for preemptive therapy of cytomegalovirus (CMV) infection. However, even at standard doses, plasma concentrations of the active metabolite ganciclovir (GCV) show substantial inter\u2010individual variability. To ensure therapeutic efficacy and minimize adverse effects, therapeutic drug monitoring (TDM) based on the area under the concentration\u2013time curve (AUC) is essential. Yet, conventional TDM via venous blood sampling is invasive and unsuitable for frequent monitoring. In this study, we aimed to develop a simple and minimally invasive method for GCV quantification using dried blood spots (DBS) combined with liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). The method demonstrated a good linearity over a concentration range of 0.25\u201316\u2009\u03bcg\/mL and satisfied the validation criteria for accuracy and precision. It showed acceptable stability for up to 7\u2009days under refrigerated conditions. Methanol was identified as the optimal extraction solvent, allowing for a simplified sample pretreatment without the need for ultrasonic processing. While hematocrit levels affected spot size and quantification accuracy, reliable measurements were obtained within the 30%\u201350% hematocrit range. The established DBS\u2010based LC\u2013MS\/MS method provides a promising, minimally invasive approach for TDM of GCV in the management of CMV infections.<\/jats:p>","DOI":"10.1002\/bmc.70411","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:41:50Z","timestamp":1772980910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a Liquid Chromatograph\u2013Tandem Mass Spectrometry Method for Quantifying Ganciclovir in Dried Blood Spots for Therapeutic Drug Monitoring"],"prefix":"10.1002","volume":"40","author":[{"given":"Naoki","family":"Tamura","sequence":"first","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"},{"name":"Department of Pharmaceutical Technology Kobe Pharmaceutical University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7982-7881","authenticated-orcid":false,"given":"Kotaro","family":"Itohara","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru","family":"Kouyama","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"},{"name":"Laboratory of Pharmacy Practice, Comprehensive Education and Research Center Kobe Pharmaceutical University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Kitahiro","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"},{"name":"Department of Integrated Clinical and Basic Pharmaceutical Sciences, Faculty of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Omura","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyasu","family":"Sakane","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology Kobe Pharmaceutical University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Saegusa","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuko","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Kobe University Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/ejhpharm\u20102022\u2010003589"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.5.1781"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.10.4063.bloodjournal88104063"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.1735"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.13.156"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199112053252303"},{"key":"e_1_2_9_8_1","unstructured":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use.2022. \u201cICH Harmonised Guideline: Bioanalytical Method Validation and Study Sample Analysis M10.\u201dhttps:\/\/database.ich.org\/sites\/default\/files\/M10_Guideline_Step4_2022_0524.pdf."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000001257"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2025.107200"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.14.298"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002191"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2016.12.008"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/339329"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab195"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000925"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab419"},{"key":"e_1_2_9_18_1","unstructured":"Mitsubishi Tanabe Pharma Corporation.2024. \u201cInterview Form for Valganciclovir (Valixa).\u201d 11th edition revised November 2024. 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