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At a flow rate of 0.6\u2009mL\/min, the mobile phase consisted of 900\u2009mL of an aqueous mixture of 0.9% diammonium hydrogen phosphate with 0.1% of 1\u2010heptane sodium sulfonate maintained at pH 3.0 and a potassium phosphate monobasic buffer solution with a pH of 3.5 mixed with 65\u2009mL acetonitrile and 35\u2009mL methanol. Chondroitin concentrations ranged from 175.1 to 3502\u2009ppm, glucosamine concentrations from 375.6 to 7512\u2009ppm, and ascorbic acid ranged from 30.2 to 603.2\u2009ppm. The detection wavelength was 190\u2009nm, the injection volume was 10\u2009\u03bcL, and the analysis runtime was less than 6.5\u2009min. The method was shown to be ecologically beneficial by the whiteness score of 97.1, AES of 84, AGSA of 69.44, CaFRI of 85, AGREEprep of 0.59, MoGAPI of 76, BAGI of 80, and CACI of 74. Ecological sustainability and alignment to ICH, EMA, and US FDA validation items have been achieved by the proposed method.<\/jats:p>","DOI":"10.1002\/bmc.70414","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T15:21:42Z","timestamp":1773328902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Eco\u2010Friendly Chromatographic Technique for Quality Control of Glucosamine, Chondroitin, and Ascorbic Acid in Both Bulk and Caplet Formulations"],"prefix":"10.1002","volume":"40","author":[{"given":"Jin\u2010Kui","family":"Ma","sequence":"first","affiliation":[{"name":"School of Food &amp; Pharmaceutical Engineering Zhaoqing University  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food &amp; Pharmaceutical Engineering Zhaoqing University  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Tong","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Food &amp; Pharmaceutical Engineering Zhaoqing University  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nai\u2010Long","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Food &amp; Pharmaceutical Engineering Zhaoqing University  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9000-3777","authenticated-orcid":false,"given":"Ahmed\u00a0Sobhy","family":"Darwish","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Science Faculty Zagazig University  Zagazig Egypt"},{"name":"EIPICO Company, Egyptian International Pharmaceutical Industries Company  10th of Ramadan Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wageh\u00a0Sobhy","family":"Darwish","sequence":"additional","affiliation":[{"name":"Food Hygiene, Safety and Technology Department, Faculty of Veterinary Medicine Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Chen","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Food &amp; Pharmaceutical Engineering Zhaoqing University  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/ejvs.2023.234808.1667"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100407"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(91)90296\u20106"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.130205"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198506980.001.0001"},{"key":"e_1_2_10_7_1","unstructured":"British Pharmacopoeia.2024. \u201cMedicines and Healthcare Products Regulatory Agency.\u201d London: The Stationery Office on Behalf of the MHRA. 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