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The objective of this study was to compare the chromatographic profiles of seven samples of copaiba oil\u2010resin with respect to chromatographic and physicochemical properties and to evaluate their biological activities. In addition, an HPLC method for quantifying \u03b2\u2010caryophyllene in the samples was developed and validated. The analysis revealed that most samples exhibited similar chromatographic profiles in TLC and HPLC\u2010DAD, while GC\u2013MS identified \u03b2\u2010caryophyllene in all the samples. The analytical method presented adequate linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.9996), with no significant matrix effect. The \u03b2\u2010caryophyllene content varied from 3.36% to 37.12% across samples. A substantial majority of samples exhibited antimicrobial activity, with similar cytotoxicity observed in RAW 264.7 cells.\n                    <jats:italic>In silico<\/jats:italic>\n                    predictions indicated a potential for toxicity in the oil\u2010resin. The results highlight the importance of physicochemical and chromatographic analyses for quality control of copaiba oil\u2010resin.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70416","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T16:46:25Z","timestamp":1773765985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Profiling and Biological Activity of Copaiba Oil\u2010Resin Samples and Development of HPLC\u2010DAD Method for the Analysis of \u03b2\u2010Caryophyllene"],"prefix":"10.1002","volume":"40","author":[{"given":"Gabriel\u00a0de\u00a0A.\u00a0P.","family":"Gra\u00e7a","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Fitoqu\u00edmica e Prospec\u00e7\u00e3o Farmac\u00eautica Funda\u00e7\u00e3o Ezequiel Dias  Belo Horizonte Minas Gerais 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