{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:18:22Z","timestamp":1776262702106,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012579","name":"Natural Science Foundation of Qinghai Province","doi-asserted-by":"publisher","award":["2023\u2010ZJ\u2010982Q"],"award-info":[{"award-number":["2023\u2010ZJ\u2010982Q"]}],"id":[{"id":"10.13039\/501100012579","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To investigate the mechanism of\n                    <jats:italic>Gentiana veitchiorum<\/jats:italic>\n                    in treating asthma using network pharmacology and untargeted metabolomics, with validation by molecular docking. LC\u2013MS\/MS was employed to comprehensively analyze metabolites in flowers of\n                    <jats:italic>G. veitchiorum<\/jats:italic>\n                    collected from Taoheyuan National Wetland Park. Asthma\u2010related compounds were retrieved from TCMSP and compared with identified metabolites. Potential targets were predicted using TCMSP and Uniprot, while asthma\u2010related targets were acquired from GeneCards. Common targets were selected to construct compound\u2010target and protein\u2013protein interaction networks using Cytoscape and STRING. Functional enrichment analysis was performed via DAVID. The top three bioactive components with the most targets were molecularly docked with the top 10 hub targets. In total, 904 metabolites were identified. Among them, 23 matched known anti\u2010asthma compounds from TCMSP. Intersection analysis revealed 388 common targets between drug and disease. Kaempferol, luteolin, and isorhamnetin showed the highest number of target associations. Molecular docking demonstrated strong binding between kaempferol and PRKACA.\n                    <jats:italic>G. veitchiorum<\/jats:italic>\n                    may treat asthma through active compounds like kaempferol, luteolin, and isorhamnetin, modulating core genes such as TP53, AKT1, IL6, TNF, and PRKACA. This study provides a scientific foundation for further clinical research and development of\n                    <jats:italic>G. veitchiorum<\/jats:italic>\n                    in treating asthma and other diseases.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70420","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:02:40Z","timestamp":1773658960000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Mechanism of\n                    <i>Gentiana veitchiorum<\/i>\n                    in Treating Asthma Through Network Pharmacology, Untargeted Metabolomics, and Molecular Docking Technology"],"prefix":"10.1002","volume":"40","author":[{"given":"Hongting","family":"Mo","sequence":"first","affiliation":[{"name":"Key Laboratory of Qinghai Province for Landscape Plants Research, Plateau Flower Research Centre Qinghai University  Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuye","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Qinghai Province for Landscape Plants Research, Plateau Flower Research Centre Qinghai University  Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiangfeng","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Qinghai Province for Landscape Plants Research, Plateau Flower Research Centre Qinghai University  Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingliang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Qinghai Province for Landscape Plants Research, Plateau Flower Research Centre Qinghai University  Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"27","article-title":"Studies on the Chemical Composition of the Mongolian Medicinal Herb Blue Yuzhan Gentian","volume":"41","author":"Bao G.","year":"2024","journal-title":"China Modern Applied Pharmacology"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116860"},{"issue":"6","key":"e_1_2_10_4_1","first-page":"601","article-title":"A Preliminary Study on the Propagation of Gentian Cuttings of the Blue Yuzan","volume":"8","author":"Chen Q.","year":"2024","journal-title":"Highland Agriculture"},{"issue":"4","key":"e_1_2_10_5_1","first-page":"1000","article-title":"Progress on the Role and Mechanism of Active Ingredients of Traditional Chinese Medicine in Regulating the Balance of Th1\/Th2 and Th17\/Treg Immune Responses in Asthma","volume":"50","author":"Deng Y.","year":"2025","journal-title":"Chinese Journal of Traditional Chinese Medicine"},{"issue":"3","key":"e_1_2_10_6_1","first-page":"1229","article-title":"Analysis of the Active Components and Their Mechanisms of the Anti\u2010Oxidative Stress Effects of Paeonia lactiflora Based on Network Pharmacology and Molecular Docking","volume":"50","author":"Du J.\u2010H.","year":"2023","journal-title":"China Animal Husbandry & Veterinary Medicine"},{"key":"e_1_2_10_7_1","first-page":"50","article-title":"Research Progress of Gentian Flavonoids in Tibetan Blue Yuzan","volume":"28","author":"Du X.","year":"2016","journal-title":"Agricultural Science and Information"},{"issue":"1","key":"e_1_2_10_8_1","first-page":"41","article-title":"Effect of Kaempferol Modulating IL\u20106\/STAT3 Signaling Pathway on Inflammatory Response in Gestational Diabetic Rats","volume":"22","author":"Guo Y.\u2010L.","year":"2025","journal-title":"Journal of Medical Molecular 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Clinical Review"},{"issue":"12","key":"e_1_2_10_14_1","first-page":"2367","article-title":"Lignans Inhibit the Proliferation of Lung Cancer A549 Cells by Increasing ROS Production and Down\u2010Regulating AKT\/mTOR Pathway and HO\u20101 Protein Expression","volume":"44","author":"Li H.","year":"2024","journal-title":"Journal of Southern Medical University"},{"issue":"11","key":"e_1_2_10_15_1","first-page":"2785","article-title":"Research Progress of Novel Programmed Cell Death in Asthma","volume":"45","author":"Li J.","year":"2024","journal-title":"Jilin Yixue"},{"key":"e_1_2_10_16_1","unstructured":"Li Y.2023. \u201cResearch on the Transplanting Technology of Gentian Cuttings From Blue Yuzan.\u201d Dissertation Tibet College of Agriculture and Animal Husbandry."},{"key":"e_1_2_10_17_1","unstructured":"Liang J. H.2024. \u201cAnalysis of the Mechanism of Action of Beimu Gua Vulgaris in the Treatment of Asthma Based on Network Pharmacology and Animal Experiments.\u201d Dissertation Heilongjiang University of Traditional Chinese Medicine."},{"issue":"14","key":"e_1_2_10_18_1","first-page":"5","article-title":"Effects of Astragalus Extract on Airway Resistance and Lung Function in Mice With Bronchial Asthma and Its Mechanism","volume":"33","author":"Lin H.","year":"2023","journal-title":"Shenzhen Journal of Integrative Chinese and Western Medicine"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb45120601"},{"issue":"5","key":"e_1_2_10_20_1","first-page":"65","article-title":"Species and Basal Origin of Tibetan Medicinal Herbs of Gentianaceae","volume":"29","author":"Nanga C.","year":"2023","journal-title":"Chinese Medicine Guide"},{"issue":"6","key":"e_1_2_10_21_1","first-page":"1047","article-title":"Research Progress on the Relationship Between Asthma and Environmental Fungi in the Chinese Population","volume":"44","author":"Shi J.","year":"2015","journal-title":"Health Research"},{"issue":"10","key":"e_1_2_10_22_1","first-page":"3352","article-title":"Progress of Chinese Medicine in Regulating Bronchial Asthma\u2010Related Signaling Pathways","volume":"46","author":"Shi M.","year":"2024","journal-title":"Chinese Patent Medicine"},{"issue":"5","key":"e_1_2_10_23_1","article-title":"A Case of Pott's Puffy Tumor in a Patient With Eosinophilic Chronic Rhinosinusitis","volume":"16","author":"Tatsuki S.","year":"2024","journal-title":"Cureus"},{"issue":"3","key":"e_1_2_10_24_1","first-page":"327","article-title":"Systematic Evaluation of the Correlation Between Asthma and Indoor Environment in Chinese Children","volume":"23","author":"Wang S.\u2010M.","year":"2023","journal-title":"Chinese Journal of Evidence\u2010Based Medicine"},{"issue":"1","key":"e_1_2_10_25_1","first-page":"46","article-title":"Progress of Research on the Role of Epidermal Growth Factor Receptor in Lung Diseases","volume":"56","author":"Wang Y.","year":"2025","journal-title":"Advances in Physiological Sciences"},{"key":"e_1_2_10_26_1","unstructured":"Xu D.2024. \u201cAnalysis of the Value of Small\u2010Dose Glucocorticoid Budesonide Inhalation in the Treatment of Bronchial Asthma.\u201d In Proceedings of the Academic Exchange Conference on Medical Nursing Innovation (Intelligent Medicine Chapter) 980\u2013982. Sichuan International Medical Exchange Promotion Association."},{"issue":"5","key":"e_1_2_10_27_1","first-page":"51","article-title":"Clinical Study and Network Pharmacological Effect Mechanism of Scutellaria Baicalensis Cough Asthma Powder in the Treatment of Bronchial Asthma","volume":"44","author":"Xu W.\u2010Y.","year":"2024","journal-title":"Modern Chinese Medicine"},{"issue":"3","key":"e_1_2_10_28_1","first-page":"729","article-title":"Effects of Ma Xing Shi Gan Tang on IL\u20106\/STAT3 Signaling Pathway and TRPV1 Receptor in Rats With Cough Variant Asthma","volume":"41","author":"Yang Q.","year":"2024","journal-title":"Journal of Guangzhou University of Traditional Chinese Medicine"},{"key":"e_1_2_10_29_1","unstructured":"Yu M.2022. \u201cResearch on the Diversity of Medicinal Plant Resources and Their Utilization in Yushu.\u201d Dissertation Northwest Agriculture and Forestry 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Journal of Asthma (Electronic Edition)"},{"issue":"2","key":"e_1_2_10_34_1","first-page":"263","article-title":"A Case of Myelodysplastic Tumor With del(5q) With TP53 Mutation Treated With Combination of Chinese and Western Medicine and Literature Review","volume":"53","author":"Zhang S.","year":"2025","journal-title":"Chinese Journal of Clinical Physicians"},{"key":"e_1_2_10_35_1","article-title":"Visualization and Analysis of Research Literature on Xiaoqinglong Tang for Bronchial Asthma Based on CiteSpace and VOSviewer","author":"Zhang X.","year":"2025","journal-title":"Chinese Journal of Chinese Medicine Library and Intelligence"},{"issue":"3","key":"e_1_2_10_36_1","first-page":"97","article-title":"Seed Germination and Healing Propagation Characteristics of the Tibetan Medicine Gentiana scabra","volume":"58","author":"Zheng X.","year":"2023","journal-title":"Journal of Gansu Agricultural University"},{"key":"e_1_2_10_37_1","first-page":"12","article-title":"Improvement of Airway 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