{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:12:14Z","timestamp":1778821934238,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mirabegron, a Beta\u20103 adrenoceptor agonist drug, and Solifenacin succinate, an antimuscarinics drug, are used to treat overactive bladder in combination. Till today, no bioanalytical method has been reported to estimate Mirabegron and Solifenacin succinate in type of matrix. Mirabegron and Solifenacin succinate were measured in rat plasma using Darifenacin as the internal standard, a newly developed and validated bioanalytical LC\u2013MS\/MS technique. The analytes were separated using a Luna C18 column and a mobile phase combination of acetonitrile: 0.1% formic acid in HPLC water (50:50 v\/v). The flow rate was maintained at an isocratic level of 1.0 mL\/min, and the runtime was about 5 min. Mirabegron (m\/z 397.51 to 83.78) and Solifenacin succinate (m\/z 481.56 to 148.79) were determined by using the mass spectra with positive mode of multiple reaction monitoring. The strategy was approved in accordance with USFDA regulations. Accuracy with mean % recovery of 96.25% to 98.45% and 96.46% to 98.05% and strong linearity with\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    value of 0.9998 in the ranges of Mirabegron (2.5\u2013100 ng\/mL) and Solifenacin succinate (0.5\u201320 ng\/mL) are the outcomes. All other parameters fall within the approved range. Indicators of medication effectiveness and safety, pharmacokinetic parameters, may be determined using the proposed technique.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70425","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:45:24Z","timestamp":1776260724000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A High\u2010Throughput LC\u2013MS\/MS Bioanalytical Method for Simultaneous Determination of Mirabegron and Solifenacin in Pharmacokinetic Investigations"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3110-4335","authenticated-orcid":false,"given":"Sadakar","family":"Tungapati","sequence":"first","affiliation":[{"name":"Analytical Development Lab, Laurus Labs Limited, R&amp;D Center  Hyderabad India"},{"name":"Division of Chemistry, Department of Sciences and Humanities Vignan's Foundation for Science, Technology and Research  Guntur Andhra Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4900-1907","authenticated-orcid":false,"given":"Srinivasu","family":"Nuvuluri","sequence":"additional","affiliation":[{"name":"Division of Chemistry, Department of Sciences and Humanities Vignan's Foundation for Science, Technology and Research  Guntur Andhra Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ramakrishna","sequence":"additional","affiliation":[{"name":"Analytical Development Lab, Laurus Labs Limited, R&amp;D Center  Hyderabad 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