{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:17:25Z","timestamp":1776262645186,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) is a leading cause of cancer\u2010related morbidity and mortality worldwide, and early detection remains essential for improving patient outcomes. This study evaluated the diagnostic potential of targeted serum nucleoside metabolomic profiling, alone and in combination with established tumor markers (CEA and CA19\u20109). Serum samples from 50 CRC patients and 25 healthy controls were analyzed using a validated LC\u2013MS\/MS platform. Sample preparation and analytical conditions were optimized using a design of experiment (DoE) approach. Multivariate analysis was performed using partial least squares\u2013discriminant analysis (PLS\u2010DA), and diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis. CRC patients showed increased purine metabolites and modified nucleosides (adenosine, inosine, xanthine, hypoxanthine, and N\n                    <jats:sup>6<\/jats:sup>\n                    \u2010methyladenosine) and decreased cytidine and uridine (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). The metabolomic panel showed strong discrimination (AUC\u2009=\u20090.91; sensitivity\u2009=\u200988%; specificity\u2009=\u200986%). Conventional markers showed moderate performance (CEA: AUC\u2009=\u20090.83; CA19\u20109: AUC\u2009=\u20090.79). Combined analysis improved performance (AUC\u2009=\u20090.95; sensitivity\u2009=\u200992%; specificity\u2009=\u200989%). Targeted serum nucleoside profiling enhances CRC detection and improves diagnostic accuracy when integrated with conventional tumor markers.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70439","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T11:19:18Z","timestamp":1775128758000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted LC\u2013MS\/MS\u2010Based Serum Nucleoside Metabolomics for Early Detection and Biomarker Discovery in Colorectal Cancer"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2517-0976","authenticated-orcid":false,"given":"Ramzy","family":"Rashed","sequence":"first","affiliation":[{"name":"College of Pharmacy Al\u2010Farahidi University  Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0849-380X","authenticated-orcid":false,"given":"Mostafa","family":"Abdelglil","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Techniques Al\u2010Farahidi University  Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0068-9616","authenticated-orcid":false,"given":"Ghada","family":"Salama","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"issue":"7","key":"e_1_2_10_3_1","first-page":"101","article-title":"Declining Bowel Cancer Incidence and Mortality in Germany: An Analysis of Time Trends in the First Ten Years After the Introduction of Screening Colonoscopy","volume":"113","author":"Brenner H.","year":"2016","journal-title":"Deutsches \u00c4rzteblatt International"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21785"},{"issue":"46","key":"e_1_2_10_5_1","first-page":"157","article-title":"Clinical Use of Tumor Biomarkers: An Overview","volume":"25","author":"Duffy M.","year":"2017","journal-title":"Klinicheskaia biokhimiia i metabolizm"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/gm341"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-1059"},{"key":"e_1_2_10_8_1","first-page":"1","volume-title":"SEER Cancer Statistics Review, 1975\u20132018","author":"Howlader 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