{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:18:41Z","timestamp":1776262721035,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A convenient and reliable SPE method coupled with a specific and sensitive LC\u2013MS\/MS technique was developed and validated for the separation and determination of unencapsulated amphotericin B (F\u2010AMB) and encapsulated amphotericin B (L\u2010AMB) in dog plasma. L\u2010AMB and F\u2010AMB in the biomatrix were simultaneously separated by Oasis HLB SPE column using natamycin as the internal standard (IS). Chromatographic separation was achieved on a ZORBAX Eclipse XDB C18 column with gradient elution at a flow rate of 0.5\u2009mL\/min. The mobile phase consisted of methanol (0.1% formic acid) and a 5\u2010mM ammonium acetate solution (0.1% formic acid). Mass spectrometry detection was performed in positive ion mode with an electrospray ionization source. Apart from regular quality control (QC) samples, a series of cross\u2010QCs was adopted to verify the specificity and reproducibility of the quantification. We showed that F\u2010AMB and L\u2010AMB were completely separated without mutual interference in the quantitative linearity ranges of F\u2010AMB and L\u2010AMB, which were 10.0\u2013800 and 100\u201325,000\u2009ng\/mL, respectively. The method was then applied to a pharmacokinetic study of liposomal amphotericin B in beagle dogs, and excellent ISR results were obtained for both L\u2010AMB and F\u2010AMB assays.<\/jats:p>","DOI":"10.1002\/bmc.70444","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T08:39:38Z","timestamp":1775723978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of Encapsulated and Unencapsulated Amphotericin B in Dog Plasma by LC\u2013MS\/MS Coupled With a Simple and Efficient Solid\u2010Phase Extraction: Application to a Pharmacokinetic Study of Liposomal Amphotericin B"],"prefix":"10.1002","volume":"40","author":[{"given":"Haiyan","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanyu","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruihan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiping","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6066-6558","authenticated-orcid":false,"given":"Xiumei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Pharmacy School Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"issue":"2","key":"e_1_2_9_2_1","first-page":"122","article-title":"Amphotericin B: An Update","volume":"53","author":"Abu\u2010Salah K. 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