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HBV\u2010Tg mice and HepG2.2.15 cells were used as in\u00a0vivo and in\u00a0vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR. We also performed untargeted metabolomics via UPLC\u2010Q\u2010TOF\/MS. Moreover, the contents of NF\u2010\u03baB pathway\u2013related genes and proteins were measured through conducting qRT\u2010PCR and Western blotting assays, and P65 nuclear translocation was examined via immunofluorescence assays. The results revealed that erythrocentaurin significantly inhibited the replication of HBV, reversed 55 differentially abundant metabolites, core\u2010regulated glutathione and arachidonic acid metabolism, and suppressed the NF\u2010\u03baB pathway by downregulating p\u2010I\u03baK\/p\u2010P65, upregulating I\u03baB, and inhibiting the nuclear translocation of P65. This study provided theoretical support and broad prospects for developing new anti\u2010HBV monomer drugs and offered a novel model that can be used for identifying pharmacodynamic substances and the secondary development of traditional Chinese medicine formulae and single herbs.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70447","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T23:56:22Z","timestamp":1776383782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Untargeted Metabolomic Study of the Anti\u2010HBV Effects of Erythrocentaurin In\u00a0Vitro and In\u00a0Vivo Using UPLC\u2010Q\u2010TOF\/MS"],"prefix":"10.1002","volume":"40","author":[{"given":"Yige","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, College of Pharmacy Heilongjiang University of Chinese Medicine  Harbin 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