{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:21:22Z","timestamp":1791652882471,"version":"4.3.4"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study elucidates the material basis of Kanli granules (KLG), a widely used Chinese herbal preparation for chronic heart failure (CHF). Using UPLC\u2010Q\u2010TOF\u2010MS\/MS, we identified 288 compounds in KLG and detected 43 constituents in rat plasma, 99 in urine, and 59 in bile. Integrated network pharmacology and literature mining screened nine key components (including paeoniflorin and atractylenolide III) responsible for the therapeutic effects. A quantitative UPLC\u2010MS\/MS method was developed for these constituents, and pharmacokinetic experiments were conducted on six compounds. All six exhibited rapid absorption (\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    &lt;\u20091\u2009h), with paeoniflorin and atractylenolide III showing significantly higher systemic exposure. Notably, the AUC of hypaconine and mangiferin was markedly greater in female rats, indicating a gender\u2010related difference in metabolism. This work provides the first comprehensive chemical composition spectrum of KLG and clarifies its potential bioactive compounds, offering new insights for the material basis research and active component screening of Chinese herbal medicine compound preparations.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70451","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T12:47:31Z","timestamp":1777898851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated Characterization and Pharmacokinetics of Kanli Granule in Rats Based on UPLC\u2010Q\u2010TOF\u2010MS\/MS: In\u00a0Vitro and In\u00a0Vivo Component Screening"],"prefix":"10.1002","volume":"40","author":[{"given":"Xuehao","family":"Cheng","sequence":"first","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Xia","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"},{"name":"Affiliated Zhongshan Hospital of Dalian University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8881-0822","authenticated-orcid":false,"given":"Xiaofen","family":"Ruan","sequence":"additional","affiliation":[{"name":"Cardiovascular Department Shuguang Hospital of Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhishan","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiutao","family":"Wang","sequence":"additional","affiliation":[{"name":"Guang'an Men Hospital China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Kang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Lifeng","sequence":"additional","affiliation":[{"name":"Cangzhou Medical College  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huinan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixian","family":"Jiang","sequence":"additional","affiliation":[{"name":"Cardiovascular Department Shuguang Hospital of Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4201-1803","authenticated-orcid":false,"given":"Yingfei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111215"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1677-x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116160"},{"issue":"2","key":"e_1_2_10_5_1","first-page":"196","article-title":"Effect of Kanli Granule on Myocardial Remodeling and Transforming Growth Factor\u2010\u03b21\/Smads Signaling Pathway in Rats With Chronic Heart Failure","volume":"36","author":"Liu Y. M.","year":"2016","journal-title":"Chinese Journal of Integrated Traditional and Western Medicine [Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi]"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-011-0756-5"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110340"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2022.03.043"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"324","article-title":"Protective Effect of Total Flavonoids From Rosa laevigata Michx on Myocardial Ischemia\u2010Reperfusion Injury in Rats","volume":"10","author":"Na M. H.","year":"2012","journal-title":"Journal of Chinese Integrative Medicine [Zhong Xi Yi Jie He Xue Bao]"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129555"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03755-0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116849"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1363678"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(21)60001-8"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200223666220630124403"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-025-00416-9"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70451","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70451","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70451","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:49:59Z","timestamp":1778820599000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,4]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/bmc.70451"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70451","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,4]]},"assertion":[{"value":"2026-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70451"}}