{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:18:21Z","timestamp":1776262701971,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Herbal adulteration has become a common malpractice, often undertaken to increase the weight or perceived potency of medicinal products or to reduce production costs. Consequently, the development of effective methods for detecting and preventing herbal adulteration is of critical importance.\n Angelica sinensis<\/jats:italic>\n root is a well\u2010known medicinal and edible plant with diverse pharmacological activities. However, owing to its increasing demand and market price, various adulterants have emerged, among which\n Angelica pubescens<\/jats:italic>\n root is one of the most prevalent. The identification of\n \n A.\u2009sinensis<\/jats:italic>\n <\/jats:styled-content>\n becomes particularly challenging when it is processed into Chinese patent medicine. To address this issue, a novel analytical strategy was proposed. Amplicon sequencing combined with gas chromatography\u2013ion mobility spectrometry was employed for the rapid screening of\n \n A.\u2009sinensis<\/jats:italic>\n <\/jats:styled-content>\n root adulteration with\n \n A.\u2009pubescens<\/jats:italic>\n <\/jats:styled-content>\n root in raw materials used for Chinese patent drugs. First, adulteration was confirmed using ITS2 amplicon sequencing. Subsequently, a simple and rapid GC\u2013IMS\u2013based screening method was established. Finally, the developed method was validated using two Chinese patent drugs to assess the presence of\n \n A.\u2009pubescens<\/jats:italic>\n <\/jats:styled-content>\n adulteration. This integrated strategy provides an effective tool for the rapid screening of commercial products and contributes to combating the adulteration of raw materials used in Chinese patent drugs.\n <\/jats:p>","DOI":"10.1002\/bmc.70454","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T02:20:06Z","timestamp":1776219606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Screening of\n Angelica sinensis<\/i>\n Root Adulteration With\n Angelica pubescens<\/i>\n Root in Raw Materials of Chinese Patent Drugs Using Amplicon Sequencing and Gas Chromatography\u2010Ion Mobility Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Wu","family":"Bi","sequence":"first","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyun","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunyou","family":"Tang","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Fang","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5127-3273","authenticated-orcid":false,"given":"Hui","family":"Sun","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Ding","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenli","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Institute for Drug Control Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05147"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002657"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121604"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418\u2010024\u201001796\u20100"},{"key":"e_1_2_9_6_1","volume-title":"Pharmacopoeia of the People's Republic of China (Volume 1)","author":"National Pharmacopoeia Commission","year":"2020"},{"key":"e_1_2_9_7_1","first-page":"158","volume-title":"Flora of China","author":"Pan Z. 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