{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:31:44Z","timestamp":1791293504830,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A combined approach of fingerprint, chemometric analysis, and network pharmacology based on available studies was employed to identify quality markers (Q\u2010markers) in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Moringa oleifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves (MOLs) for hyperglycemia. Subsequently, we conducted a quantitative analysis of these Q\u2010markers to provide a scientific basis for quality control and clinical application of MOLs. High\u2010performance liquid chromatography (HPLC) was used to establish the fingerprint of MOLs, and similarity evaluation and common peak identification were conducted. Chemometric methods were applied to screen differential biomarkers of MOLs from different batches. Based on the findings from network pharmacology, a \u201ccomponent\u2013target\u2013pathway\u201d network was constructed using the identified key targets and pathways to predict the potential hypoglycemic Q\u2010markers of MOLs. Moreover, 12 batches of MOLs were used as research objects to determine the content of Q\u2010markers. Four compounds associated with therapeutic efficacy were identified as Q\u2010markers through fingerprint analysis and a series of chemometric methods. Network pharmacology analysis was performed based on the chosen Q\u2010markers to elaborate the potential pharmacodynamic mechanisms. More importantly, it will provide a reference for further research on the quality control and pharmacological substance basis of MOLs, as well as data support for the rational utilization of MOLs medicinal materials.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70460","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:20:08Z","timestamp":1776918008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Study on Quality Markers for Hypoglycemic Effects of\n                    <scp>\n                      <i>Moringa oleifera<\/i>\n                    <\/scp>\n                    Leaves by the Integration of Network Pharmacology, Fingerprint, and Multi\u2010Index Quantitative Analysis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3316-7383","authenticated-orcid":false,"given":"Liting","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2882-8099","authenticated-orcid":false,"given":"Xuesheng","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Natural Medicine University of the Western Cape  Bellville South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7617-6831","authenticated-orcid":false,"given":"Zubing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Tropical Crop Sciences  Jinghong Yunnan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2950-8898","authenticated-orcid":false,"given":"Xiaoku","family":"Ran","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5555-7116","authenticated-orcid":false,"given":"Xiaoxia","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8035-0722","authenticated-orcid":false,"given":"Kamran","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Biology The University of Haripur (UOH)  Haripur Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0571-3416","authenticated-orcid":false,"given":"Deqiang","family":"Dou","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102944"},{"issue":"2","key":"e_1_2_11_3_1","first-page":"315","article-title":"Research Progress of Hypoglycemic Effect and Mechanism of Moringa oleifera Leaves","volume":"12","author":"Dong X. K.","year":"2019","journal-title":"Global Traditional Chinese Medicine"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3fo02640j"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.02.017"},{"key":"e_1_2_11_6_1","volume-title":"Extraction, Separation, and Purification of Moringa oleifera Leaves Flavonoids and Study of the Hypoglycemic Activity and Mechanism of Its Main Component, Isoquercitrin [D]","author":"Li J.","year":"2015"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-024-04526-x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.05.013"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546516682493"},{"issue":"11","key":"e_1_2_11_10_1","first-page":"2444","article-title":"Network Pharmacology Study and In\u00a0Vitro Experimental Verification of the Regulation of PTP1B by Isoquercitrin to Improve Insulin Resistance","volume":"37","author":"Liu S. 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