{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:13:29Z","timestamp":1778822009332,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703683"],"award-info":[{"award-number":["81703683"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Shenyanshu Tablets (SYST) is a traditional Chinese patent medicine composed of 10 kinds of Chinese herbal medicines, which has the functions of tonifying the kidney and strengthening the spleen, promoting diuresis and reducing swelling. It is well known that SYST can be used to treat chronic nephritis. However, due to the complexity of the formula, the pharmacological active components in SYST that can be absorbed into plasma have not been fully studied. In this study, an ultrahigh\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC\u2010Q\u2010TOF\u2010MS) method was first developed for separating and identifying the absorbed components and metabolites of SYST in rat plasma. A total of 33 compounds were identified or preliminarily characterized, including 18 prototype components and 15 metabolites. The results demonstrated that alkaloids, fatty acids, organic acids, and triterpenoid glycosides were the main prototype components, and triterpenoid glycoside\u2013related metabolites, fatty acid\u2013related metabolites, and flavonoid\u2010related metabolites were the major metabolites. These findings clarify the material basis of SYST in the treatment of chronic nephritis and provide references for further research and development of SYST.<\/jats:p>","DOI":"10.1002\/bmc.70472","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:01:23Z","timestamp":1777507283000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of the Absorbed Constituents and Metabolites of Shenyanshu Tablets in Rat Plasma by Ultrahigh\u2010Performance Liquid Chromatography Coupled With Quadrupole Time\u2010of\u2010Flight Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Xing","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengyu","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ChunFu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jilin Luwang Pharmaceutical Co. Ltd  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihe","family":"Wang","sequence":"additional","affiliation":[{"name":"Jilin Luwang Pharmaceutical Co. Ltd  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3945-8455","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2999-2344","authenticated-orcid":false,"given":"Lele","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renbo","family":"An","sequence":"additional","affiliation":[{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0212-0121","authenticated-orcid":false,"given":"Heyun","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.70191"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00816-y"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3902-3_21"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2023.02.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.04.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2897"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112104"},{"key":"e_1_2_8_9_1","first-page":"1669","article-title":"Clinical Study on Shenyanshu Tablets Combined With Mycohenolate Mofetil in Treatment of Chronic Nephritis","volume":"36","author":"Cheng Q.","year":"2021","journal-title":"Drugs and Clinic"},{"key":"e_1_2_8_10_1","first-page":"1046","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2025"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgr.2016.11.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3920735"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113917"},{"key":"e_1_2_8_14_1","first-page":"128","article-title":"Observation on the Curative Effect of Shenyanshu Tablets in the Treatment of Chronic Nephritis","volume":"16","author":"Gu H. 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