{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:11:55Z","timestamp":1778821915146,"version":"3.51.4"},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The Huoxiang\u2013Huanglian (HH) drug pair has demonstrated efficacy in treating enteritis, yet its underlying mechanisms remain unclear. We integrated UPLC\u2010MS, network pharmacology, and metabolomics to identify HH components and predict targets for inflammatory bowel disease (IBD). Molecular docking assessed interactions between bioactive compounds and core targets. A 2,4,6\u2010trinitrobenzenesulfonic acid (TNBS)\u2010induced zebrafish enteritis model was treated with HH (100, 200, and 400\u2009\u03bcg\/mL) or dexamethasone. Colon histopathology was evaluated via H&amp;E and AB\u2010PAS staining. Inflammatory cytokines, oxidative markers, and mRNA\/protein expression of key pathway molecules were analyzed. Integrated analysis revealed 147 potential targets and 45 differential metabolites, identifying ornithine decarboxylase 1 (ODC1) as a key target. Molecular docking confirmed strong binding (below \u22125.00\u2009kJ\/mol) of HH components like retusin and berberine to ODC1. HH treatment reduced interleukin\u20101 beta (IL\u20101\u03b2), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), malondialdehyde (MDA), NLR family pyrin domain containing 3 (NLRP3), Caspase\u20101, nuclear factor kappa B (NF\u2010\u03baB) p65, and ODC1 levels, while increasing interleukin\u201010 (IL\u201010), superoxide dismutase (SOD), and aryl hydrocarbon receptor (AhR) expression. Our findings suggest that HH alleviates IBD, which may involve the NF\u2010\u03baB\/AhR\/ODC1 pathway, highlighting its multitarget therapeutic potential.<\/jats:p>","DOI":"10.1002\/bmc.70481","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T05:21:05Z","timestamp":1778649665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ameliorative Effect and Mechanism of Huoxiang\u2013Huanglian on TNBS\u2010Induced Inflammatory Bowel Disease in Zebrafish"],"prefix":"10.1002","volume":"40","author":[{"given":"Yanan","family":"Wang","sequence":"first","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahao","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang 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