{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:54:50Z","timestamp":1781765690856,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cistanoside A (Cis A) has received more attention because of its potential in the treatment of osteoporosis. However, its in\u00a0vivo process remains unknown. In this study, a simple liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) method was developed and validated for the determination of cistanoside A in rat plasma, urine, and feces. After prepared by protein precipitation, cistanoside A and IS were separated on a Waters BEH C18 column with MS detection operated in positive multiple reaction monitoring mode. The method was fully validated and applied to the pharmacokinetic and excretion studies of cistanoside A in rats after intravenous (5\u2009mg\/kg) and oral (10, 20, and 40\u2009mg\/kg) administration. After intravenous administration, cistanoside A showed moderate clearance (0.52\u2009\u00b1\u20090.04\u2009L*h\/kg), low volume of distribution, and short terminal half\u2010life (0.43\u2009\u00b1\u20090.03\u2009h). After oral administration at doses of 10, 20, and 40\u2009mg\/kg, the system exposure increased lower than the increase of dose, and the oral bioavailabilities were 0.39%, 0.38%, and 0.23%, respectively. After oral administration, the average cumulative excretion rate was 0.03% in urine and 1.62% in feces at 24\u2009h. In summary, this study defined the pharmacokinetic and excretion characteristics of cistanoside A in rats, which could provide useful information for its further development.<\/jats:p>","DOI":"10.1002\/bmc.70508","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T04:46:59Z","timestamp":1780375619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pharmacokinetic, Bioavailability, and Excretion Study of Cistanoside A Following Intragastric and Intravenous Administration in Rats Using Ultra\u2010High Performance Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Jingjing","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pharmacy Wujing Community Health Service Center, Minhang District  Shanghai 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