{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:53:32Z","timestamp":1781765612122,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903765"],"award-info":[{"award-number":["81903765"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Huachansu (HCS) tablets, prepared from dried toad skin through water decoction and ethanol extraction, possess detoxifying and analgesic effects and are clinically used in the treatment of advanced tumors. However, the in\u00a0vivo metabolism of HCS tablets has not been systematically investigated. In this study, a comprehensive analysis strategy based on ultra\u2010performance liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry (UPLC\u2013ESI\u2013QTOF\/MS\n                    <jats:sup>E<\/jats:sup>\n                    ) was established for screening and identifying the prototype components and metabolites of HCS tablets in rat urine and feces. A total of 28 prototype components (16 bufadienolides, six alkaloids, four amino acids and peptides, two nucleosides) and 64 metabolites (44 bufadienolide\u2010related metabolites and 20 alkaloid\u2010related metabolites) were identified in the urine and feces of rats treated with HCS tablets. Based on the analysis of 64 metabolites, the metabolic processes of HCS tablets in rats mainly include desaturation, reduction, dehydroxylation, hydroxylation, hydration, dehydration, glucuronidation, methylation, acetylcysteine conjugation, acetylcysteine\u2010S conjugation, and other related conjugation reactions. This approach enabled the rapid identification of HCS\u2010related exogenous components in rat urine and feces, helping to elucidate the active substances and facilitate mechanistic research.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70514","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:03:01Z","timestamp":1780570981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Comprehensive Strategy for Characterizing Metabolites and Metabolic Profile in Rat Urine and Feces Following Oral Administration of Huachansu Tablets Based on UPLC\u2013ESI\u2013QTOF\/MS\n                    <sup>E<\/sup>"],"prefix":"10.1002","volume":"40","author":[{"given":"Yongzhi","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijuan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1909-4316","authenticated-orcid":false,"given":"Hui","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Huarun Jinchan Pharmaceutical Co. 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