{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:54:23Z","timestamp":1781765663314,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A rapid and sensitive LC\u2013MS\/MS method was developed and validated for quantifying 6\u2010TG and 6\u2010MMP concentrations in erythrocytes. This method is applicable for routine therapeutic drug monitoring in inflammatory bowel disease patients using thiopurines. Red blood cell (RBC) count was performed after washing the blood samples. After storage at \u221220\u00b0C, protein precipitation followed by acidic hydrolysis was performed using 0.062\u2009mol\/L DTT and 1.36\u2009mol\/L perchloric acid with an incubation time of 60\u2009min at 100\u00b0C. Chromatographic separation was performed on an Acquity UPLC HSS T3 column (2.1\u2009\u00d7\u2009100\u2009mm, 1.8\u2009\u03bcm) using ammonium formate 5\u2009mmol\/L in 0.1% aqueous formic acid and methanol in gradient elution at a flow rate of 0.4\u2009mL\/min. Detection was performed in multiple reaction monitoring mode using positive electrospray ionization. The method was linear over a calibration range of 25\u20131500\u2009pmol\/100\u2009\u03bcL for 6\u2010TG and 250\u20138000\u2009pmol\/100\u2009\u03bcL for 6\u2010MMP. The method demonstrated good performance in terms of intraday and interday accuracy (96.2%\u2013110.8%) and precision (CV 0.9%\u20133.9%). Calibration and QC samples were stable for 3\u2009months at \u221280\u00b0C. The method demonstrated excellent performance in proficiency testing.<\/jats:p>","DOI":"10.1002\/bmc.70515","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T01:45:21Z","timestamp":1781142321000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Optimized Sample Pretreatment Method Followed by a Validated LC\u2013MS\/MS Method for Quantifying 6\u2010TG and 6\u2010MMP Concentrations in Red Blood Cells for Therapeutic Drug Monitoring in Inflammatory Bowel Disease Patients Using Thiopurines"],"prefix":"10.1002","volume":"40","author":[{"given":"Inge R. 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