{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:54:23Z","timestamp":1781765663075,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Paeoniae Radix Alba (PRA, roots of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Paeonia lactiflora<\/jats:italic>\n                    <\/jats:styled-content>\n                    Pall.) yields stir\u2010baked (sbPRA), charred (cPRA), and carbonized (caPRA) specifications through increasing degrees of stir\u2010baking. In this study, an ultrahigh\u2010performance liquid chromatography (UPLC) method was developed to quantitatively determine seven chemical constituents in 15 batches of PRA with varying degrees of stir\u2010baking. The method showed excellent linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009\u2265\u20090.9995), with precision RSDs &lt;\u20092%, repeatability and stability RSDs of 0.93%\u20132.8% and 0.68%\u20131.5%, respectively, and mean recoveries of 92.24%\u2013105.9% (RSD &lt;\u20093%). In caPRA, most constituents were below the detection limit. Oxypaeoniflorin, catechin, paeoniflorin, and benzoylpaeoniflorin decreased progressively with increasing processing intensity, whereas gallic acid increased markedly from PRA to cPRA. The concentrations of albiflorin and 1,2,3,4,6\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010pentagalloylglucose showed an initial increase followed by a decrease, reaching maximum levels in sbPRA. Partial least squares discriminant analysis (PLS\u2010DA) identified gallic acid as a key discriminatory marker. Simulated processing experiments showed that paeoniflorin and benzoylpaeoniflorin could decompose into benzoic acid, while 1,2,3,4,6\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010pentagalloylglucose could degrade into gallic acid. This study compared the content of seven components in PRA subjected to different degrees of stir\u2010baking and clarified their transformation patterns.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70518","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T01:36:14Z","timestamp":1781487374000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UPLC Multiwavelength Quantification and PLS\u2010DA Reveal Stir\u2010Baking Degree\u2010Dependent Marker Dynamics in Paeoniae Radix Alba"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6818-5433","authenticated-orcid":false,"given":"Lixia","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"},{"name":"Research Centre for Traditional Chinese Medicine of Lingnan (Southern China) Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5073-3206","authenticated-orcid":false,"given":"Weiting","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"},{"name":"Research Centre for Traditional Chinese Medicine of Lingnan (Southern China) Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonglin","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinju","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"},{"name":"Research Centre for Traditional Chinese Medicine of Lingnan (Southern China) Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Centre for Traditional Chinese Medicine of Lingnan (Southern China) Jinan University  Guangzhou China"},{"name":"National Engineering Research Centre for Modernization of Traditional Chinese Medicine Lingnan Resources Branch  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghua","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"},{"name":"Guangdong Key Lab of Traditional Chinese Medicine Information Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-8246","authenticated-orcid":false,"given":"Zhiguo","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou China"},{"name":"Guangdong Key Lab of Traditional Chinese Medicine Information Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7318-2165","authenticated-orcid":false,"given":"Hui","family":"Cao","sequence":"additional","affiliation":[{"name":"Research Centre for Traditional Chinese Medicine of Lingnan (Southern China) Jinan University  Guangzhou China"},{"name":"National Engineering Research Centre for Modernization of Traditional Chinese Medicine Lingnan Resources Branch  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,14]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Integration of National Traditional Chinese Medicine Processing Experience and Norms (Updated Version)","author":"Cao H.","year":"2017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2023.04.016"},{"key":"e_1_2_9_4_1","volume-title":"Pharmacopoeia of the People's Republic of China (Part I)","author":"Chinese Pharmacopoeia Commission","year":"2025"},{"key":"e_1_2_9_5_1","volume-title":"Hubei Province Standard for Processing of Chinese Herbal Decoction Pieces (2018 Edition)","author":"Drug Administration of Hubei Province","year":"2018"},{"key":"e_1_2_9_6_1","volume-title":"Shandong Province Processing Specification of Traditional Chinese Medicine Decoction Pieces (2022 Edition)","author":"Drug Administration of Shandong Province & Shandong Institute of Food and Drug Inspection","year":"2022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.156037"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/1047322X.1990.10389587"},{"key":"e_1_2_9_9_1","unstructured":"Jiang D. L.2025. \u201cStudy on Quality Analysis of Stir\u2010Fried Radix Paeoniae Alba With Different Degrees of Concoctions Based on Electrosensory\u2010Chemical Composition\u2010Machine Learning.\u201d [Master's Thesis Anhui University of Chinese Medicine]."},{"key":"e_1_2_9_10_1","first-page":"3564","article-title":"UPLC Fingerprint of Paeoniae Alba Radix From Different Regions","volume":"46","author":"Jin L.","year":"2015","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5621"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117319"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115897"},{"key":"e_1_2_9_14_1","first-page":"16","article-title":"Quality Comparative Study on Radix Paeoniae Alba and Its Processed Products by HPLC Characteristic Chromatogram Combined With Content Determination","volume":"34","author":"Liang Y. Y.","year":"2022","journal-title":"Natural Product Research and Development"},{"key":"e_1_2_9_15_1","first-page":"980","article-title":"Determination of Bioactive Components in Paeonia lactiflora Roots Cultivated in Various Areas by UHPLC","volume":"39","author":"Liu Y. Q.","year":"2016","journal-title":"Chinese Medicinal Materials"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.09.105"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5653"},{"key":"e_1_2_9_18_1","first-page":"2048","article-title":"Composition Differences of Different Processed Paeoniae Radix Alba Based on Fingerprint and Chemometrics","volume":"19","author":"Ren J.","year":"2024","journal-title":"Shijie Zhongyiyao"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117372"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155519"},{"key":"e_1_2_9_21_1","first-page":"1118","article-title":"WANG Pei's Experience in Treating Bladder Cancer","volume":"63","author":"Wang J.","year":"2022","journal-title":"Journal of Traditional Chinese Medicine"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4901"},{"key":"e_1_2_9_23_1","first-page":"2909","article-title":"Effect of Processing on the Contents of Nine Components in Paeoniae Radix Alba","volume":"43","author":"Yu D. R.","year":"2020","journal-title":"Chinese Medicinal Materials"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010023\u201004570\u20105"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S281976"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70518","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:20:53Z","timestamp":1781763653000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,14]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/bmc.70518"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70518","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,14]]},"assertion":[{"value":"2026-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70518"}}