{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:26:35Z","timestamp":1790130395622,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Analytical performance in anti\u2010doping science continues to evolve through the identification of novel metabolites, improved detection sensitivity, and the use of alternative biological matrices. In recent years, dried blood spots (DBS) have gained increasing attention in doping control due to advantages such as improved analyte stability and simplified, cost\u2010effective sample collection, shipment, and storage. However, DBS sampling is inherently limited by the small sample volume and the restricted number of spots available for analysis. Therefore, sensitive and comprehensive analytical strategies are required to maximize the information obtained from a single DBS or Tasso M20 devices. This study aimed to develop and validate a two\u2010step analytical workflow enabling the detection of 91 target compounds, including selected non\u2010threshold substances (NTS) and anabolic steroid esters (ASE), in two dried blood matrices: cellulose\u2010based DBS cards and volumetric absorptive microsampling devices (Tasso M20 pebbles). The protocol comprises extraction with a methanol\u2013acetonitrile mixture (4:1, v\/v), followed by LC\u2013MS\/MS analysis for NTS. Subsequently, the extract is evaporated, derivatized with Girard P reagent, and analyzed by LC\u2013MS\/MS for ASE detection. The validated method proved suitable for qualitative detection of the selected prohibited substances.<\/jats:p>","DOI":"10.1002\/bmc.70531","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T13:15:51Z","timestamp":1782393351000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dried Blood Spots for Doping Purpose\u2014Two\u2010Step Protocol for Analysis of Non\u2010Threshold Substances and Anabolic Steroid Esters From One Spot\/Pebble"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-6190","authenticated-orcid":false,"given":"B.","family":"Stojanovic","sequence":"first","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Pecher","sequence":"additional","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Jeitler","sequence":"additional","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Portenschlager","sequence":"additional","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Geisendorfer","sequence":"additional","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Gmeiner","sequence":"additional","affiliation":[{"name":"Seibersdorf Labor GmbH  Seibersdorf Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5633"},{"key":"e_1_2_10_3_1","unstructured":"ISO.2017. \u201cISO\/IEC 17025: General Requirements for the Competence of Testing and Calibration Laboratories\u201d."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3226"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3889"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3379"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3660"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020361s"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c01417"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2025.117048"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3914"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3681"},{"key":"e_1_2_10_13_1","volume-title":"Toxics (Vol. 13, Number 1)","author":"Pires B.","year":"2025"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.117890"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3030"},{"key":"e_1_2_10_16_1","unstructured":"Stojanovic B. T.Geisendorfer V.Jeitler S.Illedits andG.Gmeiner.2022. \u201cCapability and Stability of Dried Blood Spots for Doping Analysis Recent Advances in Doping Analysis.\u201d InProceedings of the Manfred Donike Workshop 40th Cologne Workshop on Dope Analysis.89\u201391."},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3950"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2022.2103085"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.5188"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.03.013"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.18176\/archmeddeporte.00156"},{"key":"e_1_2_10_22_1","unstructured":"WADA.2021a. \u201cDried Blood Spots (DBS) for Doping Control.\u201dhttps:\/\/www.wadaama.org\/sites\/default\/files\/item_6_3_3_attach_1_finalversion_td2021dbs_final.pdf."},{"key":"e_1_2_10_23_1","unstructured":"WADA.2021b. \u201cInternational Standard for Laboratories 2021.\u201dhttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/resurces\/files\/isl_2021.pdf."},{"key":"e_1_2_10_24_1","unstructured":"WADA.2023. \u201cWADA Technical Document\u2010TD2023DBS Document Number: TD2023DBS Version Number: 1.0 DRIED BLOOD SPOTS (DBS) FOR DOPING CONTROL Requirements and Procedures for Analytical Testing and Storage.\u201dhttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2022\u201011\/td2023dbs_cleanversion.pdf."},{"key":"e_1_2_10_25_1","unstructured":"WADA.2026a. \u201cDried Blood Spots (DBS) for Doping Control Requirements and Procedures for Analytical Testing and Sample Storage.\u201dhttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2025\u201009\/td2026dbs_finaleng.pdf."},{"key":"e_1_2_10_26_1","unstructured":"WADA.2026b. \u201cThe 2026 Prohibited List World Anti\u2010Doping Code.\u201dhttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2025\u201009\/2026list_en_final_clean_september_2025.pdf."},{"key":"e_1_2_10_27_1","unstructured":"WADA.2026c. \u201cThe Prohibited List2026.\u201dwww.wada\u2010ama.org."},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123535"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70531","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70531","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70531","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T13:38:01Z","timestamp":1786023481000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,25]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/bmc.70531"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70531","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,25]]},"assertion":[{"value":"2026-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70531"}}