{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:41:03Z","timestamp":1786117263557,"version":"build-2736575974"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dasatinib (DAS) is a tyrosine kinase inhibitor used in the treatment of chronic myeloid leukemia (CML) and erythromycin (ERY) is macrolide antibiotic used to treat a range of inflammatory conditions are often co\u2010administered in clinical practice. The simultaneous estimation of DAS and ERY helps in understanding the pharmacokinetics and drug\u2013drug interactions. In this respect, we presented novel, sensitive, and validated bio\u2010analytical LC\u2010ESI\u2010MS\/MS method for simultaneous estimation of DAS and ERY in CD\u20101 mice plasma. The LLOQ was 2.0\u2009ng\/mL with an S\/N ratio of 15. This validated method was used to study the PK of DAS and ERY in mice following intravenous and oral administration. The DAS was administered at 5 mg\/kg\u2009via both oral and intraveneous route, whereas ERY was administered at 50 mg\/kg orally and 5 mg\/kg intraveneousaly. Following intravenous dosing, DAS showed high clearance and distribution, while ERY exhibited high clearance and distribution. The oral bioavailability was 64% for DAS and 21% for ERY. When co\u2010administered orally at 5\u2009mg\/kg for DAS and 50\u2009mg\/kg of ERY, the\n                    <jats:italic>AUC<\/jats:italic>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    of DAS were unchanged. However, the systemic exposure (w.r.t\n                    <jats:italic>AUC<\/jats:italic>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    ) of ERY was slightly higher (2.4\u2010fold) in the presence of DAS, which may be attributed because of the CYP3A4 inhibition potential.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70533","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T15:47:43Z","timestamp":1783698463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Simple and Sensitive LC\u2013MS\/MS Method for Simultaneous Quantification of Dasatinib and Erythromycin in Mice Plasma: Application to Preclinical Pharmacokinetic Study"],"prefix":"10.1002","volume":"40","author":[{"given":"Avinash","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Chemistry Pacific Academy of Higher Education and Research University (PAHER)  Udaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirbhay Kumar","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Pharmacokinetic Department Aurigene Oncology Limited  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Giri","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Pharmacokinetic Department Aurigene Oncology Limited  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh","family":"Patnam","sequence":"additional","affiliation":[{"name":"Prochem Organics Private Limited  Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rameshwar","family":"Ameta","sequence":"additional","affiliation":[{"name":"Department of Chemistry Pacific Academy of Higher Education and Research University (PAHER)  Udaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2009.09.019"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(20000215)14:3<156::AID-RCM850>3.0.CO;2-K"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/s2175-97902023e21415"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.121851"},{"key":"e_1_2_6_6_1","unstructured":"DHHS FDA CDER &CVM2018Guidance for Industry: Bioanalytical Method ValidationUS Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research and Center for Veterinary Medicine"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.11.005"},{"key":"e_1_2_6_8_1","unstructured":"Food and Drug Administration. n.d. \u201cBioanalytical Method: Validation Guidance for Industry.\u201dhttps:\/\/www.fda.gov\/files\/drugs\/published\/Bioanalytical\u2010Method\u2010Validation\u2010Guidance\u2010for\u2010Industry.pdf."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-007-0478-8"},{"key":"e_1_2_6_10_1","volume-title":"Pharmacokinetic evaluation Dasatanib and Erythromycin in CD1 Mice","author":"BioRender","year":"2026"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(97)00095-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt.1434"},{"key":"e_1_2_6_13_1","first-page":"868","volume-title":"VeterinaryPharmacology and Therapeutics","author":"Papich M. 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