{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:41:48Z","timestamp":1786117308652,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["310291\/2021\u20116"],"award-info":[{"award-number":["310291\/2021\u20116"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008994","name":"Universidade de Bras\u00edlia","doi-asserted-by":"publisher","award":["004\/2025"],"award-info":[{"award-number":["004\/2025"]}],"id":[{"id":"10.13039\/100008994","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study validates a selective, robust high\u2010performance liquid chromatography (HPLC) method for the simultaneous determination of bergenin (BER) and alpha\u2010lipoic acid (ALA) in skin permeation studies. Separation used a Discovery C\n                    <jats:sub>18<\/jats:sub>\n                    column (250\u2009\u00d7\u20094.6\u2009mm, 5\u2009\u03bcm) and a mobile phase of acetonitrile and aqueous phosphoric acid (pH\u20093.0). A multistep gradient (10%\u201330% acetonitrile from 0\u20132\u2009min; 30%\u201340% from 2\u20136\u2009min; and 40%\u201360% from 6\u20139\u2009min) at 1.0\u2009mL\/min enabled a 20\u2010min run time with UV detection at 210\u2009nm. Following ICH M10 bioanalytical guidelines, the method showed linearity (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009&gt;\u20090.999) from 0.5 to 15\u2009\u03bcg\/mL for both analytes. Limits of detection were 0.120 and 0.083\u2009\u03bcg\/mL, and limits of quantification were 0.363 and 0.250\u2009\u03bcg\/mL for BER and ALA, respectively. Precision and accuracy were satisfactory, with a relative standard deviation (RSD)\u2009&lt;\u20093.5%, while recoveries ranged between 80.8% and 96.2% (RSD\u2009&lt;\u200910.0%) from porcine stratum corneum and viable skin. The method proved robust against variations in temperature, flow rate, and column batch, without endogenous skin interference. In conclusion, this validated method is a reliable tool for supporting the development of topical formulations containing the association of BER and ALA.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70538","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:53:42Z","timestamp":1782791622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chromatographic Method Validation for Simultaneous Determination of Bergenin and Alpha\u2010Lipoic Acid in Skin Penetration Studies"],"prefix":"10.1002","volume":"40","author":[{"given":"Samuel","family":"Dutra\u2010Portes","sequence":"first","affiliation":[{"name":"Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia  Bras\u00edlia Distrito Federal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara S.","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia  Bras\u00edlia Distrito Federal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breno N.","family":"Matos","sequence":"additional","affiliation":[{"name":"Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia  Bras\u00edlia Distrito Federal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6481-2364","authenticated-orcid":false,"given":"Tais","family":"Gratieri","sequence":"additional","affiliation":[{"name":"Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia  Bras\u00edlia Distrito Federal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-6852","authenticated-orcid":false,"given":"Marcilio","family":"Cunha\u2010Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia  Bras\u00edlia Distrito 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