{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:42:15Z","timestamp":1786117335805,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      This study aimed to develop and validate a liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) assay for the simultaneous quantification of pregabalin (PGB) and gabapentin (GBP) and to explore real\u2010world pharmacokinetic variability. A bioanalytical LC\u2013MS\/MS method was validated using serum samples prepared by acetonitrile protein precipitation. Chromatographic separation was achieved on a C18 column with gradient elution and positive electrospray ionization. The validated method was applied to serum samples obtained from 65 patients, and associations between serum drug concentrations and clinical measures were evaluated. The assay demonstrated excellent linearity (\n                      <jats:italic>R<\/jats:italic>\n                      \u00b2 &gt; 0.998), acceptable precision and accuracy, recovery ranging from 96.9% to 101.5%, and matrix effects below 7%. Considerable inter\u2010individual variability in serum concentrations was observed (PGB: median 700 ng\/mL [IQR: 500\u20131000]; GBP: median 4500 ng\/mL [IQR: 3200\u20135700]). No significant dose\u2013concentration relationships were identified (PGB:\n                      <jats:italic>\u03c1<\/jats:italic>\n                      = 0.03; GBP:\n                      <jats:italic>\u03c1<\/jats:italic>\n                      = \u22120.08), and no significant associations were observed between serum concentrations and the clinical outcome measures assessed. In contrast, both drugs showed moderate inverse correlations with post\u2010dose sampling interval (PGB:\n                      <jats:italic>\u03c1<\/jats:italic>\n                      = \u22120.46; GBP:\n                      <jats:italic>\u03c1<\/jats:italic>\n                      = \u22120.45). These findings indicate substantial pharmacokinetic variability of gabapentinoids under routine clinical conditions. Although no clear dose\u2013concentration or exposure\u2013response relationships were identified in this cohort, interpretation should be made cautiously because of non\u2010standardized sampling conditions and the relatively narrow range of renal function among participants.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/bmc.70539","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:49:08Z","timestamp":1783057748000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LC\u2013MS\/MS\u2010Based Monitoring of Pregabalin and Gabapentin Reveals Marked Pharmacokinetic Variability and Limited Exposure\u2013Response Relationships in Neuropathic Pain Patients"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8564-1824","authenticated-orcid":false,"given":"Duygu","family":"Eryavuz Onmaz","sequence":"first","affiliation":[{"name":"Department of Biochemistry Bandirma Onyedi Eylul University Faculty of Medicine  Bandirma Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6834-0827","authenticated-orcid":false,"given":"Fettah","family":"Eren","sequence":"additional","affiliation":[{"name":"Department of Neurology Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kadir","family":"Gulsen","sequence":"additional","affiliation":[{"name":"Department of Neurology Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firdevs","family":"Sak","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ahmad","family":"Bik","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"Coban","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sedat","family":"Abusoglu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Unlu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Selcuk University Faculty of Medicine  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations8020021"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1556\/1326.2023.01104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468\u20101331.2010.02999.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2003.12.024"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167\u20106296(01)00130\u20108"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737175.2024.2385947"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph18020151"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.12.030"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2049463720912496"},{"key":"e_1_2_10_11_1","volume-title":"Liquid Chromatography\u2013Mass Spectrometry Methods; Approved Guideline (CLSI Document C62\u2010A)","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2014"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkz070"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13122045"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265\u2010020\u201001432\u20107"},{"key":"e_1_2_10_15_1","volume-title":"Bioanalytical Method Validation: Guidance for Industry","author":"Food and Drug Administration (FDA)","year":"2018"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.21608\/mjfmct.2015.47317"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2019.0086"},{"key":"e_1_2_10_18_1","first-page":"74","article-title":"Does Pregabalin Affect Renal Functions and Plasma Electrolytes","volume":"4","author":"Gunay A. 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