{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:42:18Z","timestamp":1786117338834,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["PL2024H197"],"award-info":[{"award-number":["PL2024H197"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    While flavonoids from\n                    <jats:italic>Cuscutae Semen<\/jats:italic>\n                    (CS) exert significant estrogen\u2010regulating effects, their intestinal metabolism under estrogen\u2010deficient conditions remains poorly understood. This study aimed to elucidate the metabolic fate of these active constituents. Using ovariectomized rats, we collected ileal sac fluid, colonic sac fluid, hepatic portal blood, and feces to establish metabolic profiles along the \u201cileum\u2010colon\u2010hepatic portal vein\u2010feces\u201d axis via UPLC\u2010Q\u2010TOF\u2010MS. By employing a combined approach to evaluate both mucosa\u2010mediated and microbiota\u2010mediated metabolism, we identified 37 parent compounds (predominantly flavonoids) and 48 metabolites. The ileum and colon were confirmed as primary metabolic sites, predominantly involving Phase I and Phase II reactions. Metabolic pathways were detailed using quercetin as a representative phytoestrogen. Furthermore, we analyzed the intestinal metabolic patterns of nine in\u00a0vivo direct\u2010acting compounds highly correlated with CS's estrogenic effects. Collectively, this study uncovers the intestinal metabolic characteristics of CS active fractions under estrogen deficiency, providing a crucial foundation for future in\u00a0vivo studies and elucidating the pharmacologically active basis of CS.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70543","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:01:27Z","timestamp":1783569687000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intestinal Metabolic Pathways and Key Metabolites of Phytoestrogenic Fractions From Cuscutae Semen in Estrogen\u2010Deficient Rats: Insights From UPLC\u2010Q\u2010TOF MS"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6996-2418","authenticated-orcid":false,"given":"Mingyao","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Pharmacy Harbin University of Commerce  Harbin 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