{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:40Z","timestamp":1786692400702,"version":"build-2736575974"},"reference-count":14,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A sensitive and practical liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) method was developed and validated for the quantification of HY\u2010078020 in human plasma. Plasma samples (100\u2009\u03bcL) were processed by liquid\u2013liquid extraction with methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether using HY\u2010078020\u2010d6 as the internal standard. Chromatographic separation was achieved on a Phenomenex Luna C8(2) column with gradient elution, and detection was performed in positive electrospray ionization mode using multiple reaction monitoring. The monitored transitions were m\/z 515.2\u2009\u2192\u2009282.3 for HY\u2010078020 and m\/z 521.1\u2009\u2192\u2009282.3 for the internal standard. The assay was validated over the concentration range of 4.0\u20132400\u2009ng\/mL with a lower limit of quantification of 4.0\u2009ng\/mL. The method showed acceptable selectivity, accuracy, precision, matrix effect, extraction recovery, carryover, and stability in accordance with current bioanalytical validation criteria. Intra\u2010 and interbatch precision was within 9.03% and 5.46%, respectively, and the analyte remained stable under benchtop, freeze\u2013thaw, processed\u2010sample, reinjection, and long\u2010term storage conditions. With its small plasma volume requirement, broad calibration range, low carryover, and robust stability, this method is suitable for routine quantification of HY\u2010078020 in human plasma and may support future pharmacokinetic studies.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70549","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:36:25Z","timestamp":1784507785000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of an LC\u2013MS\/MS Method for the Quantification of HY\u2010078020 in Human Plasma Using Liquid\u2013Liquid Extraction and Its Application to Human Pharmacokinetic Study"],"prefix":"10.1002","volume":"40","author":[{"given":"Yaqi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The Second Affiliated Hospital of Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yazhu","family":"Cui","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Shao","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linying","family":"Meng","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqin","family":"Chen","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"An","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Shen","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3347-7449","authenticated-orcid":false,"given":"Zhaoxing","family":"Chu","sequence":"additional","affiliation":[{"name":"Hefei Industrial Pharmaceutical Institute Co. Ltd.  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