{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:40:59Z","timestamp":1786117259696,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fulzerasib is a novel, potent, and irreversible covalent inhibitor targeting the KRAS G12C mutation, recently approved in China for advanced nonsmall cell lung cancer. This study aimed to develop and fully validate a rapid, specific and sensitive UHPLC\u2010MS\/MS method for the quantification of Fulzerasib in rat plasma. Sample preparation was streamlined using a simple protein precipitation technique with acetonitrile. Chromatographic separation was achieved within a 4.0\u2010min run on a C18 column, with verapamil as the internal standard and detection via multiple reaction monitoring. The method was rigorously validated over a linear range of 5 to 5000\u2009ng\/mL (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009&gt;\u20090.9933), demonstrating excellent accuracy (102%\u2013112.7%) and precision (\u2264\u200910.3% for QCs). Stability was confirmed under various storage and processing conditions. The validated method was successfully applied in the first pharmacokinetic study of Fulzerasib in Sprague\u2013Dawley rats following single intravenous (1\u2009mg\/kg) and oral (3\u2009mg\/kg) administration. Fulzerasib exhibited favorable pharmacokinetic properties, including low plasma clearance (5.02\u2009mL\/min\/kg), moderate volume of distribution and notably high oral bioavailability of 38.6%. The established method is reliable, efficient and readily applicable, thereby providing a vital tool for accelerating the ongoing preclinical and translational research of Fulzerasib.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70552","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:55:46Z","timestamp":1784084146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a Sensitive UHPLC\u2013MS\/MS Analytical Method for Fulzerasib in Rat Plasma and its Application to Pharmacokinetics Studies"],"prefix":"10.1002","volume":"40","author":[{"given":"Qiang","family":"Liu","sequence":"first","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Zhu","family":"Zhao","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Sheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong","family":"Lan","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Lu","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0486-4829","authenticated-orcid":false,"given":"Hong\u2010Can","family":"Ren","sequence":"additional","affiliation":[{"name":"GenFleet Therapeutics (Shanghai) Inc  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2025.102957"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"1","article-title":"An Innovative Stability\u2010Indicating Liquid Chromatography With Tandem Mass Spectrometry Method Development and Validation for the Determination of Sotorasib in Human Plasma","volume":"86","author":"Bysani S. 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