{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:42:12Z","timestamp":1786117332946,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515010405"],"award-info":[{"award-number":["2023A1515010405"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274120"],"award-info":[{"award-number":["82274120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    SHR6508, a novel calcimimetic agent, is currently being developed for treating secondary hyperparathyroidism in chronic kidney disease (CKD) patients requiring maintenance hemodialysis. For the purpose of quantifying SHR6508 in human plasma, a sensitive and reliable LC\u2013MS\/MS method was first developed and successfully validated. Since SHR6508 is a disulfide bond\u2010containing peptide, ascorbic acid was supplemented into blood samples during collection to prevent thiol\u2010disulfide exchange reactions. Plasma matrix samples were processed by protein precipitation with a water\u2013acetonitrile solution (1:4, v\/v) containing 0.05% formic acid. Chromatographic separation was conducted on an XBridge Peptide BEH C18 column (4.6\u2009\u00d7\u2009100\u2009mm, 5.0\u2009\u03bcm) under gradient elution with water and methanol, each containing 0.1% trifluoroacetic acid. SHR6508 was quantified by tandem mass spectrometry in positive electrospray ionization using multiple reaction monitoring with transitions of\n                    <jats:italic>m\/z<\/jats:italic>\n                    532.0\u2009\u2192\u2009455.5 for SHR6508 and\n                    <jats:italic>m\/z<\/jats:italic>\n                    537.0\u2009\u2192\u2009456.5 for SHR180296 (isotope\u2010labelled internal standard). The quantification linear range was 1.00\u20131000\u2009ng\/mL. All validation parameters including selectivity, carryover, precision, accuracy, recovery, and matrix effect fell within the predefined acceptance criteria. The stabilization strategy developed in this work may also offer valuable insights for the collection and analysis of other disulfide bond\u2010containing peptide drugs.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70555","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:49:07Z","timestamp":1783756147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Overcoming Stability Challenge During the Quantification of SHR6508 in Human Plasma by LC\u2013MS\/MS"],"prefix":"10.1002","volume":"40","author":[{"given":"Yuan","family":"Wu","sequence":"first","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"},{"name":"Department of Clinical Pharmacy, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6721-469X","authenticated-orcid":false,"given":"Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"Suzhou Haike Medical Technology Co. Ltd.  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Suzhou Haike Medical Technology Co. Ltd.  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6531-2046","authenticated-orcid":false,"given":"Laiyou","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"},{"name":"Department of Clinical Pharmacy, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744\u20109987.13434"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880903200203"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2215\/cjn.06040710"},{"key":"e_1_2_9_5_1","first-page":"747","volume-title":"Vitamin D (Third Edition)","author":"David V.","year":"2011"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.11.112"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfq219"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/dddt.s134103"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2017.08.026"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744\u20109987.12772"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000414"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088\u2010200948050\u201000002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2020.02.439"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154\u2010017\u20109421\u20104"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.115.068007"},{"key":"e_1_2_9_16_1","unstructured":"U.S. Department of Health and Human Services; Food and Drug Administration; Center for Drug Evaluation and Research (CDER); Center for Veterinary Medicine (CVM).2018. \u201cGuidance for Industry Bioanalytical Method Validation.\u201dhttps:\/\/www.fda.gov\/media\/70858\/download."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22696"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2147\/dddt.s160223"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1059828"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210\u2010024\u201003705\u20104"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70555","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70555","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70555","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T13:38:25Z","timestamp":1786023505000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,11]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/bmc.70555"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70555","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,11]]},"assertion":[{"value":"2026-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70555"}}