{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:38Z","timestamp":1786692398003,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012131","name":"Department of Science and Technology of Liaoning Province","doi-asserted-by":"publisher","award":["2025\u2010BS\u20100759"],"award-info":[{"award-number":["2025\u2010BS\u20100759"]}],"id":[{"id":"10.13039\/501100012131","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Simvastatin (ST) has limited long\u2010term clinical utility due to its adverse effects. The combination of Hedan tablets and simvastatin (HST) exhibits superior lipid\u2010lowering effects, but its mechanism remains unclear. This study aims to investigate the effects of HST in hyperlipidemic rats based on 16S rRNA gene sequencing and metabolomics. Gut microbiota composition was analyzed by 16S rRNA sequencing; fecal short\u2010chain fatty acids (SCFAs) and bile acids (BAs) in the liver and serum were quantified by GC\u2010MS and LC\u2010MS, respectively; and hepatic BA metabolism\u2013related gene expression was detected by RT\u2010qPCR. 16S rRNA sequencing revealed that HST influenced gut microbiota composition and increased microbial diversity. Compared with ST, the effect of HST on SCFA levels was primarily reflected in elevated propionic acid and isovaleric acid levels, with total SCFA content significantly higher than that in the MOD group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). RT\u2010qPCR results showed that HST\u2010mediated BA regulation involved the upregulation of hepatic BA synthesis genes (CYP8B1 and BACS) and the transport gene (MRP2), as well as the downregulation of reabsorption genes (NTCP and ASBT) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). This study suggests that the ameliorative effect of HST on hyperlipidemia is associated with the regulation of gut microbiota and its metabolites (SCFAs and BAs).\n                  <\/jats:p>","DOI":"10.1002\/bmc.70559","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:45:55Z","timestamp":1785249955000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Hedan Tablets Combined With Simvastatin on Hyperlipidemia: Insights From Microbiomics and Metabolomics"],"prefix":"10.1002","volume":"40","author":[{"given":"Rongrong","family":"Gao","sequence":"first","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Pharmaceutical University  Shenyang Liaoning 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