{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:40:04Z","timestamp":1788280804715,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study optimized the freeze\u2010drying process of Scorpio, compared its chemical and pharmacodynamic equivalence with traditional salt\u2010processed Scorpio in treating CIA model rats, established an equivalent conversion relationship, and evaluated acute toxicity. Single\u2010factor experiments and orthogonal tests were conducted with prefreezing time, drying time, and temperature as factors and water\u2010soluble protein content, ethanol\u2010soluble extract content, and freeze\u2010drying rate as indices. The analytic hierarchy process\u2010criteria importance through intercriteria correlation (AHP\u2010CRITIC) mixed weighting method was used for comprehensive scoring to determine the optimal process. Chemical equivalence was calculated using four amino acids and water\u2010soluble protein content. Pharmacodynamic equivalence was evaluated in a complete Freund's adjuvant\u2010induced arthritis rat model using arthritis index, rheumatoid factor, and serum inflammatory cytokines (IL\u20106, TNF\u2010\u03b1, and IL\u20101\u03b2). Acute toxicity was assessed by determining the LD\n                    <jats:sub>5<\/jats:sub>\n                    <jats:sub>0<\/jats:sub>\n                    . Results showed that freeze\u2010killing pretreatment was superior to scalding. The optimal process was prefreezing for 2\u2009h, drying at 45\u00b0C for 16\u2009h. Chemical equivalence indicated that 1\u2009g of freeze\u2010dried Scorpio was equivalent to 1.455\u2009g of salt\u2010processed Scorpio. Pharmacodynamic equivalence showed that 1\u2009g of freeze\u2010dried Scorpio was equivalent to 1.081\u2009g of salt\u2010processed Scorpio. The LD\n                    <jats:sub>5<\/jats:sub>\n                    <jats:sub>0<\/jats:sub>\n                    of freeze\u2010dried Scorpio was 11.091\u2009g\/kg.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70562","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:48:38Z","timestamp":1785336518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integration of HPLC Amino Acid Profiling With AHP\u2010CRITIC Guided Freeze\u2010Drying Process Optimization, Chemical\u2013Pharmacodynamic Equivalence, and Acute Toxicity Evaluation of Scorpio"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7309-2232","authenticated-orcid":false,"given":"Wan","family":"Ziteng","sequence":"first","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Xiao","sequence":"additional","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhen","sequence":"additional","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Xiang","sequence":"additional","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Qi","sequence":"additional","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Xingxing","sequence":"additional","affiliation":[{"name":"Jiangxi University of Chinese Medicine  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu","family":"Jinrong","sequence":"additional","affiliation":[{"name":"Jiangxi Tianyuan Pharmaceutical Co. Ltd  Yichun Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu","family":"Jinhong","sequence":"additional","affiliation":[{"name":"Jiangxi Tianyuan Pharmaceutical Co. 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