{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:37Z","timestamp":1786692397848,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pharmaceutical and cosmetic preservatives play a critical role in inhibiting microbial contamination, preventing chemical degradation, and maintaining product efficacy and safety throughout the shelf life. Given their functional importance, comparable to that of active pharmaceutical ingredients, it is essential to monitor their quality using advanced analytical techniques. Among the most commonly employed seven varieties of preservatives in industry are 2,4\u2010dihydroxybenzoic acid, 4\u2010hydroxybenzoic acid, 3,4\u2010dihydroxybenzoic acid, benzyl alcohol, benzaldehyde, phenol, sodium benzoate, methylparaben, and propylparaben, either individually or in combination. The present study focuses on the development and validation of a stability\u2010indicating, reverse\u2010phase high\u2010performance liquid chromatography method for simultaneous quantification of these seven preservatives and their impurities, benzaldehyde and phenol, a total of nine components. The method demonstrates high selectivity, sensitivity, accuracy, and precision, and meets the validation criteria outlined in International Council for Harmonization (ICH) guidelines. Optimal chromatographic resolution was achieved using a Phenomenex Luna C18 column, dual\u2010wavelength UV detection at 254 and 230\u2009nm, and a gradient mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile. The method was established within a range of 1.5\u201345.0\u03bcg\/mL concentration for all components. The validated method is suitable for routine quality control testing across various pharmaceutical and cosmetic formulations.<\/jats:p>","DOI":"10.1002\/bmc.70579","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T10:02:11Z","timestamp":1785578531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Robust Stability\u2010Indicating RP\u2010HPLC Method for Simultaneous Determination of Widely Used Pharmaceutical and Cosmetic Preservatives"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0756-5081","authenticated-orcid":false,"given":"Ramanjaneyulu","family":"Seemaladinne","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Applied Sciences and Humanities Vignan's Foundation for Science, Technology and Research  Guntur Andhra Pradesh India"},{"name":"Kashiv Biosciences  Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2192-4750","authenticated-orcid":false,"given":"Sudip","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Applied Sciences and Humanities Vignan's Foundation for Science, Technology and Research  Guntur Andhra Pradesh India"},{"name":"Dr. LARA Centre for Energy Materials Vignan's Foundation for Science, Technology and Research  Guntur Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.3030420802"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2016.03.019"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.14775"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094\u2010019\u201002038\u20104"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.7b00374"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5800"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","unstructured":"Cosmetic Microbiology.2020.https:\/\/doi.org\/10.1201\/9780429113697.","DOI":"10.1201\/9780429113697"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70429"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12249\u2010017\u20100875\u20101"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","unstructured":"Davidson P. M. T. M.Taylor andJ. R. D.David.2020. \u201cAntimicrobials in Food.\u201dhttps:\/\/doi.org\/10.1201\/9780429058196.","DOI":"10.1201\/9780429058196"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2015.1087964"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.70217"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/der.0000000000000429"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40521\u2010016\u20100102\u20104"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1091581817728996"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5152"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574886317666220919121532"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/17103568251386038"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3917"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.61554\/ijnrph.v3i2.2025.206"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20976"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3202"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5944"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma16134793"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.61882\/jommid.13.2.78"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.46956\/ijihd.v10i1.656"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","unstructured":"Sharma S. S.Batra K.Nawani K.Sharma andP.Kumar.2025. \u201cRole of Preservatives in Ophthalmic Products.\u201d In Complex Ophthalmic Dosage Forms: Advances in Biomedical Applications and Future Perspectives. pp.143\u2013160.https:\/\/doi.org\/10.1007\/978\u2010981\u201096\u20106306\u20104_6.","DOI":"10.1007\/978-981-96-6306-4_6"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","unstructured":"Srivastava N. 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