{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:42Z","timestamp":1786692402267,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to elucidate the therapeutic effect and mechanism of Zhuangdu Quhan Mixture (ZDQHM) in ankylosing spondylitis (AS). An AS mouse model was established in mice using \u03b2\u2010glucan. Therapeutic efficacy was assessed through arthritis scores, histopathology, serum cytokine measurement by ELISA, and bone metabolism\u2010related protein analysis via Western blot. Active compounds were identified by UHPLC\u2013MS, with targets predicted using network pharmacology and validated by molecular docking and molecular dynamics simulations. ZDQHM significantly ameliorated spinal inflammation and histopathological damage, reduced serum IL\u20106 and TNF\u2010\u03b1 levels, and modulated bone metabolism proteins. Network pharmacology identified 45 intersecting targets between ZDQHM compounds and AS, predominantly enriched in IL\u201017, Toll\u2010like receptor, and NOD\u2010like receptor pathways. Molecular docking and dynamics demonstrated binding of key compounds to IL\u201017A. In\u00a0vivo validation revealed that ZDQHM suppressed IL\u201017A, Act1, and IL\u201017RA expression, and recombinant IL\u201017A partially reversed its therapeutic effects. ZDQHM alleviates inflammation and abnormal bone metabolism in AS mice, potentially through inhibition of the IL\u201017 signaling pathway. Beyond confirming the established role of IL\u201017 in AS, this study provides an integrated pharmacological framework explaining how ZDQHM exerts its effects via regulation of the IL\u201017A\/IL\u201017RA\/Act1 axis, offering mechanistic insights and experimental support for TCM in AS therapy.<\/jats:p>","DOI":"10.1002\/bmc.70586","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:34:06Z","timestamp":1785494046000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zhuangdu Quhan Mixture Alleviates Ankylosing Spondylitis in Mice by Inhibiting the Interleukin\u201017 Signaling"],"prefix":"10.1002","volume":"40","author":[{"given":"Dan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Rheumatology The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4491-4361","authenticated-orcid":false,"given":"Yanrong","family":"Wei","sequence":"additional","affiliation":[{"name":"Office of Clinical Trial Institutions The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijing","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqian","family":"Deng","sequence":"additional","affiliation":[{"name":"Graduate School Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6756-1683","authenticated-orcid":false,"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5497-9604","authenticated-orcid":false,"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Medicine The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.52559"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3370"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(13)61134\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1505066"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201015\u2010293"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00914"},{"key":"e_1_2_11_8_1","first-page":"576","article-title":"Clinical Study on Long\u2010Term Treatment of Ankylosing Spondylitis With Integrated Traditional Chinese and Western Medicine","volume":"21","author":"Cao T. 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