{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:42Z","timestamp":1786692402270,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Colorectal cancer (CRC) arises via the stepwise adenoma\u2013carcinoma sequence (ACS). Gut microbial dysbiosis and host metabolic reprogramming jointly correlate with CRC onset and advancement, yet their stage\u2010specific crosstalk across ACS remains largely unclear. Limited multi\u2010omics research on microbial\u2013metabolic interactions throughout ACS hinders the development of early diagnostic biomarkers and preventive strategies. Here, we combined untargeted mucosal metabolomics and fecal shotgun metagenomic sequencing in 36 participants, covering healthy controls, ACS, and CRC patients. We systematically analyzed microbial composition, functions, differential metabolites, and enriched pathways and integrated multi\u2010omics data to screen stage\u2010specific signatures. Distinct gut microbial profiles and progressive functional shifts toward pathogenicity and abnormal carbohydrate metabolism were observed along ACS. Mucosal metabolism was continuously disrupted, with prominent alterations in taurine\u2010hypotaurine, sphingolipid, and bile acid pathways. Core differential metabolites showed excellent diagnostic performance. Microbe\u2013metabolite interactions were progressively enhanced to form a concerted pro\u2010tumor axis. This study characterizes unique ACS\u2010stage microbial\u2013metabolic features. Dysregulated metabolic pathways and key microbe\u2013metabolite crosstalk are closely associated with CRC progression, offering novel non\u2010invasive biomarkers and premalignant intervention targets.<\/jats:p>","DOI":"10.1002\/bmc.70588","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:42:31Z","timestamp":1785930151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Metabolomic and Metagenomic Profiling Reveals Distinct Microbial\u2013Metabolic Signatures in the Adenoma\u2013Carcinoma Sequence of Colorectal Cancer"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8557-5836","authenticated-orcid":false,"given":"Haifan","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqi","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongying","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxin","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xiamen Haicang Hospital  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4847"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/18735592MTEx9NTk2y"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-025-00777-9"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7528"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.119732"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.612826"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.11.027"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.119"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2024.106212"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0189-8"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.14526"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abp9960"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2024.09.005"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0MO00176G"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201022666210910094827"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1AN02239C"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-09054-2"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-016-1469-y"},{"issue":"5","key":"e_1_2_13_20_1","first-page":"4834","article-title":"Characteristics of Fecal Gut Microbiota in Patients With Colorectal Cancer at Different Stages and Different Sites","volume":"18","author":"Sheng Q.","year":"2019","journal-title":"Oncology Letters"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.70424"},{"issue":"11","key":"e_1_2_13_22_1","article-title":"Gut Microbiota and Colorectal Cancer Development: A Closer Look to the Adenoma\u2010Carcinoma Sequence","volume":"8","author":"Vacante M.","year":"2020","journal-title":"Biomedicine"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7935917"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI154944"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-023-00766-x"}],"container-title":["Biomedical 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